ArticleApplied and environmental microbiology2014
Sustainable growth of Dehalococcoides mccartyi 195 by corrinoid salvaging and remodeling in defined lactate-fermenting consortia.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 75 citations in OpenAlex.
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- Skin-associatedmSphere · 2025Article
- Electron bifurcation and fluoride efflux systems implicated in defluorination of perfluorinated unsaturated carboxylic acids byScience advances · 2024Article
- Sulfonamide Per- and Polyfluoroalkyl Substances Can Impact Microorganisms Used in Aromatic Hydrocarbon and Trichloroethene Bioremediation.Environmental science & technology · 2024Article
- Skin-associatedbioRxiv : the preprint server for biology · 2024Article
- Phylogenetic distribution and experimental characterization of corrinoid production and dependence in soil bacterial isolates.The ISME journal · 2024Article
- Understanding the sources, function, and irreplaceable role of cobamides in organohalide-respiring bacteria.Frontiers in microbiology · 2024Review
- Direct Cobamide Remodeling via Additional Function of Cobamide Biosynthesis Protein CobS from Vibrio cholerae.Journal of bacteriology · 2021Article
- Interspecies metabolite transfer and aggregate formation in a co-culture of Dehalococcoides and Sulfurospirillum dehalogenating tetrachloroethene to ethene.The ISME journal · 2021Article
- Molecular characterization of microbial communities in a peat-rich aquifer system contaminated with chlorinated aliphatic compounds.Environmental science and pollution research international · 2021Article
- Organohalide-Respiring Bacteria at the Heart of Anaerobic Metabolism in Arctic Wet Tundra Soils.Applied and environmental microbiology · 2021Article
- Reconstruction and evaluation of oil-degrading consortia isolated from sediments of hydrothermal vents in the South Mid-Atlantic Ridge.Scientific reports · 2021Article
- Sharing vitamins: Cobamides unveil microbial interactions.Science (New York, N.Y.) · 2020Review
- Multi-faceted approaches to discovering and predicting microbial nutritional interactions.Current opinion in biotechnology · 2020Review
- Flexible Cobamide Metabolism inJournal of bacteriology · 2020Article
- Genetic Analysis ofFrontiers in microbiology · 2020Article
- Synthetic microbial consortia for biosynthesis and biodegradation: promises and challenges.Journal of industrial microbiology & biotechnology · 2019Review
- Uneven distribution of cobamide biosynthesis and dependence in bacteria predicted by comparative genomics.The ISME journal · 2019Article
- Transient OMicrobiome · 2018Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Corrinoids are essential cofactors of reductive dehalogenases in Dehalococcoides mccartyi, an important bacterium in bioremediation, yet sequenced D. mccartyi strains do not possess the complete pathway for de novo corrinoid biosynthesis. Pelosinus sp. and Desulfovibrio sp. have been detected in dechlorinating communities enriched from contaminated groundwater without exogenous cobalamin corrinoid. To investigate the corrinoid-related interactions among key members of these communities, we constructed consortia by growing D. mccartyi strain 195 (Dhc195) in cobalamin-free, trichloroethene (TCE)- and lactate-amended medium in cocultures with Desulfovibrio vulgaris Hildenborough (DvH) or Pelosinus fermentans R7 (PfR7) and with both in tricultures. Only the triculture exhibited sustainable dechlorination and cell growth when a physiological level of 5,6-dimethylbenzimidazole (DMB), the lower ligand of cobalamin, was provided. In the triculture, DvH provided hydrogen while PfR7 provided corrinoids to Dhc195, and the initiation of dechlorination and Dhc195 cell growth was highly dependent on the growth of PfR7. Corrinoid analysis indicated that Dhc195 imported and remodeled the phenolic corrinoids produced by PfR7 into cobalamin in the presence of DMB. Transcriptomic analyses of Dhc195 showed the induction of the CbiZ-dependent corrinoid-remodeling pathway and BtuFCD corrinoid ABC transporter genes during corrinoid salvaging and remodeling. In contrast, another operon annotated to encode a putative iron/cobalamin ABC transporter (DET1174-DET1176) was induced when cobalamin was exogenously provided. Interestingly, a global upregulation of phage-related genes was observed when PfR7 was present. These findings provide insights into both the gene regulation of corrinoid salvaging and remodeling in Dhc195 when it is grown without exogenous cobalamin and microbe-to-microbe interactions in dechlorinating microbial communities.
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