ArticleApplied and environmental microbiology2014
Two new xylanases with different substrate specificities from the human gut bacterium Bacteroides intestinalis DSM 17393.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 53 citations in OpenAlex.
- Environmental, socioeconomic, and health factors associated with gut microbiome species and strains in isolated Honduras villages.Cell reports · 2024Article
- Gut microbiota predicts the diagnosis of celiac disease in Saudi children.World journal of gastroenterology · 2023Article
- Tutorial: Microbiome studies in drug metabolism.Clinical and translational science · 2022Article
- Biochemical Basis of Xylooligosaccharide Utilisation by Gut Bacteria.International journal of molecular sciences · 2022Article
- Characterization of a novel multidomain CE15-GH8 enzyme encoded by a polysaccharide utilization locus in the human gut bacterium Bacteroides eggerthii.Scientific reports · 2021Article
- Potential Mechanisms Linking Food-Derived MicroRNAs, Gut Microbiota and Intestinal Barrier Functions in the Context of Nutrition and Human Health.Frontiers in nutrition · 2021Review
- High-Throughput Generation of Product Profiles for Arabinoxylan-Active Enzymes from Metagenomes.Applied and environmental microbiology · 2020Article
- Cloning of novel bacterial xylanases from lignocellulose-enriched compost metagenomic libraries.AMB Express · 2019Article
- Mode of Action of GH30-7 Reducing-End Xylose-Releasing Exoxylanase A (Xyn30A) from the Filamentous Fungus Talaromyces cellulolyticus.Applied and environmental microbiology · 2019Article
- Prebiotics from Seaweeds: An Ocean of Opportunity?Marine drugs · 2019Review
- Biochemical characterization of a novel exo-oligoxylanase fromBiotechnology for biofuels · 2019Article
- A New Group of Modular Xylanases in Glycoside Hydrolase Family 8 from Marine Bacteria.Applied and environmental microbiology · 2018Article
- Polysaccharide Utilization Loci: Fueling Microbial Communities.Journal of bacteriology · 2017Review
- Galactomannan Catabolism Conferred by a Polysaccharide Utilization Locus of Bacteroides ovatus: ENZYME SYNERGY AND CRYSTAL STRUCTURE OF A β-MANNANASE.The Journal of biological chemistry · 2017Article
- Effect of humic acid on anaerobic digestion of cellulose and xylan in completely stirred tank reactors: inhibitory effect, mitigation of the inhibition and the dynamics of the microbial communities.Applied microbiology and biotechnology · 2017Article
- Bacterial xylanases: biology to biotechnology.3 Biotech · 2016Review
- Bacteroides intestinalis DSM 17393, a member of the human colonic microbiome, upregulates multiple endoxylanases during growth on xylan.Scientific reports · 2016Article
- The Glycoside Hydrolase Family 8 Reducing-End Xylose-Releasing Exo-oligoxylanase Rex8A from Paenibacillus barcinonensis BP-23 Is Active on Branched Xylooligosaccharides.Applied and environmental microbiology · 2016Article
- Assessment of Fecal Microflora Changes in Pigs Supplemented with Herbal Residue and Prebiotic.PloS one · 2015Article
- The devil lies in the details: how variations in polysaccharide fine-structure impact the physiology and evolution of gut microbes.Journal of molecular biology · 2014Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Xylan is an abundant plant cell wall polysaccharide and is a dominant component of dietary fiber. Bacteria in the distal human gastrointestinal tract produce xylanase enzymes to initiate the degradation of this complex heteropolymer. These xylanases typically derive from glycoside hydrolase (GH) families 10 and 11; however, analysis of the genome sequence of the xylan-degrading human gut bacterium Bacteroides intestinalis DSM 17393 revealed the presence of two putative GH8 xylanases. In the current study, we demonstrate that the two genes encode enzymes that differ in activity. The xyn8A gene encodes an endoxylanase (Xyn8A), and rex8A encodes a reducing-end xylose-releasing exo-oligoxylanase (Rex8A). Xyn8A hydrolyzed both xylopentaose (X5) and xylohexaose (X6) to a mixture of xylobiose (X2) and xylotriose (X3), while Rex8A hydrolyzed X3 through X6 to a mixture of xylose (X1) and X2. Moreover, rex8A is located downstream of a GH3 gene (xyl3A) that was demonstrated to exhibit β-xylosidase activity and would be able to further hydrolyze X2 to X1. Mutational analyses of putative active site residues of both Xyn8A and Rex8A confirm their importance in catalysis by these enzymes. Recent genome sequences of gut bacteria reveal an increase in GH8 Rex enzymes, especially among the Bacteroidetes, indicating that these genes contribute to xylan utilization in the human gut.
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