ArticleScientific reports2014
Slowing down single-molecule trafficking through a protein nanopore reveals intermediates for peptide translocation.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- Probing Voltage Dependence Interaction of Cationic Peptides with Bacterial Porins at A Single-Molecule Level.Cell biochemistry and biophysics · 2026Article
- Nanopore-Based, Real-Time Single-Molecule Probing of i-Motif Structural Dynamics and Targeted PNA Disruption.Nano letters · 2026Article
- Vectorial Discrimination of Small Molecules with a Macrocycle Adaptor-Protein Nanopore System and Nanocavity-Dependent, pH Gradient-Controlled Analyte Kinetics.Analytical chemistry · 2025Article
- Large and Stable Nanopores Formed by Complement Component 9 for Characterizing Single Folded Proteins.ACS nano · 2025Article
- Controlling DNA Fragments Translocation across Nanopores with the Synergic Use of Site-Directed Mutagenesis, pH-Dependent Charge Tuning, and Electroosmotic Flow.ACS applied materials & interfaces · 2024Article
- Enhanced Pulley Effect for Translocation: The Interplay of Electrostatic and Hydrodynamic Forces.Biomacromolecules · 2023Article
- Detection of Biological Molecules Using Nanopore Sensing Techniques.Biomedicines · 2023Review
- Long-lived metastable knots in polyampholyte chains.PloS one · 2023Article
- Aerolysin nanopore-based identification of proteinogenic amino acids using a bipolar peptide probe.Nanoscale advances · 2022Article
- Ab Initio Properties of Hybrid Cove-Edged Graphene Nanoribbons as Metallic Electrodes for Peptide Sequencing via Transverse Tunneling Current.ACS omega · 2022Article
- Protein Sequencing, One Molecule at a Time.Annual review of biophysics · 2022Review
- Nanopore sensing: A physical-chemical approach.Biochimica et biophysica acta. Biomembranes · 2021Review
- Biological Nanopores: Engineering on Demand.Life (Basel, Switzerland) · 2021Review
- Lysenin Channels as Sensors for Ions and Molecules.Sensors (Basel, Switzerland) · 2020Review
- Application of Solid-State Nanopore in Protein Detection.International journal of molecular sciences · 2020Review
- Engineering and Modeling the Electrophoretic Trapping of a Single Protein Inside a Nanopore.ACS nano · 2019Article
- Vibration assisted electron tunneling through nano-gaps in graphene nano-ribbons for amino-acid and peptide bond recognition.Nanoscale advances · 2019Article
- Channel from bacterial virus T7 DNA packaging motor for the differentiation of peptides composed of a mixture of acidic and basic amino acids.Biomaterials · 2019Article
- Mechanism of action of antimicrobial peptide P5 truncations against Pseudomonas aeruginosa and Staphylococcus aureus.AMB Express · 2019Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The microscopic details of how peptides translocate one at a time through nanopores are crucial determinants for transport through membrane pores and important in developing nano-technologies. To date, the translocation process has been too fast relative to the resolution of the single molecule techniques that sought to detect its milestones. Using pH-tuned single-molecule electrophysiology and molecular dynamics simulations, we demonstrate how peptide passage through the α-hemolysin protein can be sufficiently slowed down to observe intermediate single-peptide sub-states associated to distinct structural milestones along the pore, and how to control residence time, direction and the sequence of spatio-temporal state-to-state dynamics of a single peptide. Molecular dynamics simulations of peptide translocation reveal the time- dependent ordering of intermediate structures of the translocating peptide inside the pore at atomic resolution. Calculations of the expected current ratios of the different pore-blocking microstates and their time sequencing are in accord with the recorded current traces.
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