Evidence map›Paper›PMID 24463015›Full record

ArticleAnalytical biochemistry2014

Bioorthogonal click chemistry to assay mu-opioid receptor palmitoylation using 15-hexadecynoic acid and immunoprecipitation.

Brittany Ebersole, Jessica Petko, Robert Levenson

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical biochemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.4field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Brittany EbersoleProgram in Chemical Biology, Penn State College of Medicine, Hershey, PA 17033, USA; Department of Pharmacology, Penn State College of Medicine, Hershey, PA 17033, USA. Electronic address: bae154@psu.edu.
Jessica PetkoDepartment of Pharmacology, Penn State College of Medicine, Hershey, PA 17033, USA.
Robert LevensonDepartment of Pharmacology, Penn State College of Medicine, Hershey, PA 17033, USA.
Pennsylvania State University · US

Funding

Mu Opioid Receptor in AddictionP20DA025995 · NIDA · UNIVERSITY OF PENNSYLVANIA · PI BERRETTINI, WADE H · 2008 to 2011
$3.4M
NIDA NIH HHS DA025995NIDA NIH HHS P20 DA025995
6 · The paper itself

Abstract

We have developed a modification of bioorthogonal click chemistry to assay the palmitoylation of cellular proteins. This assay uses 15-hexadecynoic acid (15-HDYA) as a chemical probe in combination with protein immunoprecipitation using magnetic beads in order to detect S-palmitoylation of proteins of interest. Here we demonstrate the utility of this approach for the mu-opioid receptor (MOR), a G-protein-coupled receptor (GPCR) responsible for mediating the analgesic and addictive properties of most clinically relevant opioid agonist drugs. This technique provides a rapid, non-isotopic, and efficient method to assay the palmitoylation status of a variety of cellular proteins, including most GPCRs.

Indexed as

ImmunoblottingAntibodiesClick ChemistryFatty Acids, UnsaturatedHEK293 CellsHumansImmunomagnetic SeparationImmunoprecipitationLipoylationReceptors, Opioid, muRecombinant Fusion Proteins15-hexadecynoic acidAntibodiesFatty Acids, UnsaturatedReceptors, Opioid, muRecombinant Fusion ProteinsClick chemistryImmunoprecipitationMu-opioid receptorPalmitoylation

Identifiers

PMID24463015
PMCPMC3969759
OpenAlexW2057168970

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.