ArticlePhytochemistry2014
High level protein-purification allows the unambiguous polypeptide determination of latent isoform PPO4 of mushroom tyrosinase.
Article in Phytochemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 50 citations in OpenAlex.
- Tyrosinase Recovered from White Button Mushroom Waste: Extraction, Characterization, and Application in Casein Cross-Linking.Journal of agricultural and food chemistry · 2026Article
- Tyrosinases: a family of copper-containing metalloenzymes.Chemtexts · 2024Article
- Metabolic Pathway Reconstruction Indicates the Presence of Important Medicinal Compounds inInternational journal of molecular sciences · 2023Article
- Improving Theaflavin-3,3'-digallate Production Efficiency Optimization by Transition State Conformation of Polyphenol Oxidase.Molecules (Basel, Switzerland) · 2023Article
- Biochemical characterization of Dimocarpus longan polyphenol oxidase provides insights into its catalytic efficiency.Scientific reports · 2022Article
- Two Hybrid Histidine Kinases Involved in the Ethylene Regulation of the Mycelial Growth and Postharvest Fruiting Body Maturation and Senescence of Agaricus bisporus.Microbiology spectrum · 2022Article
- Wells-Dawson phosphotungstates as mushroom tyrosinase inhibitors: a speciation study.Scientific reports · 2021Article
- Similar but Still Different: Which Amino Acid Residues Are Responsible for Varying Activities in Type-III Copper Enzymes?Chembiochem : a European journal of chemical biology · 2021Review
- Article
- Identification of Amino Acid Residues Responsible for C-H Activation in Type-III Copper Enzymes by Generating Tyrosinase Activity in a Catechol Oxidase.Angewandte Chemie (International ed. in English) · 2020Article
- Physicochemical properties of free and immobilized tyrosinase from different species of yam (Biotechnology reports (Amsterdam, Netherlands) · 2020Article
- Lectins from the Edible MushroomMolecules (Basel, Switzerland) · 2020Review
- A Peptide-Induced Self-Cleavage Reaction Initiates the Activation of Tyrosinase.Angewandte Chemie (International ed. in English) · 2019Article
- Keggin-type polyoxotungstates as mushroom tyrosinase inhibitors - A speciation study.Scientific reports · 2019Article
- Biochemical and structural characterization of tomato polyphenol oxidases provide novel insights into their substrate specificity.Scientific reports · 2019Article
- Polymerizing Like Mussels Do: Toward Synthetic Mussel Foot Proteins and Resistant Glues.Angewandte Chemie (International ed. in English) · 2018Article
- Total Synthesis, Stereochemical Assignment, and Divergent Enantioselective Enzymatic Recognition of Larreatricin.Chemistry (Weinheim an der Bergstrasse, Germany) · 2018Article
- Purification and Characterization of Latent Polyphenol Oxidase from Apricot (Prunus armeniaca L.).Journal of agricultural and food chemistry · 2017Article
- Three recombinantly expressed apple tyrosinases suggest the amino acids responsible for mono- versus diphenolase activity in plant polyphenol oxidases.Scientific reports · 2017Article
- Ten Good Reasons for the Use of the Tellurium-Centered Anderson-Evans Polyoxotungstate in Protein Crystallography.Accounts of chemical research · 2017Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Tyrosinases catalyze two initial reaction steps in the formation of melanin. Purification of tyrosinases had always been a process accompanied with various problems caused by enzymatic browning processes. Here, an approach is presented for the purification of the latent enzyme from mushrooms which averts and removes interfering compounds (e.g. polyphenols) in advance to the extraction process. The described method is supposed being well suitable as a general protein purification protocol from natural sources like fungi and plants. The purified enzyme was investigated in detail by means of mass spectrometry: its intact protein mass was determined as 64,247.3 Da and it was identified as number four of in total six isoforms (PPO1-6) by means of sequence analysis. Some PTMs, strain specific sequence disparities and several cleavage sites including the one causing enzyme-activation (Ser³⁸³) were determined, thus, providing insights on the maturation process of this latent tyrosinase zymogen. Based on these sequence data it can be concluded that the polypeptide backbone of the latent form of the tyrosinase PPO4 ranges from Ser² to Thr⁵⁶⁵, missing when compared to the gene-derived sequence a small part (46 amino acids) of the C-terminal tail. The high content on hydrophobic amino acids within this missing tail gives rise to speculations whether this part might have a function as a membrane anchor.
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