ArticleVeterinary immunology and immunopathology2014
Functional characterisation of bovine TLR5 indicates species-specific recognition of flagellin.
Article in Veterinary immunology and immunopathology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 24 citations in OpenAlex.
- Polymorphism in the TLR Adaptors of the Toll Signalling Pathway for Use in Livestock Breeding for Health Traits.International journal of molecular sciences · 2026Review
- Article
- Host and Species-Specificities of Pattern Recognition Receptors Upon Infection WithFrontiers in cellular and infection microbiology · 2022Review
- Extracellular vesicles produced by primary human keratinocytes in response to TLR agonists induce stimulus-specific responses in antigen-presenting cells.Cellular signalling · 2021Article
- Introduction of a plasmid and a protein into bovine and swine cells by water-in-oil droplet electroporation.The Journal of veterinary medical science · 2020Article
- Escape of TLR5 Recognition byFrontiers in immunology · 2020Article
- Toxoplasma gondii profilin does not stimulate an innate immune response through bovine or human TLR5.Innate immunity · 2018Article
- Functional expression of TLR5 of different vertebrate species and diversification in intestinal pathogen recognition.Scientific reports · 2018Article
- Host species adaptation of TLR5 signalling and flagellin recognition.Scientific reports · 2017Article
- The efficacy of alcelaphine herpesvirus-1 (AlHV-1) immunization with the adjuvants EmulsigenVeterinary microbiology · 2016Article
- Investigating the contribution of IL-17A and IL-17F to the host response during Escherichia coli mastitis.Veterinary research · 2015Article
- Functional analysis of bovine TLR5 and association with IgA responses of cattle following systemic immunisation with H7 flagella.Veterinary research · 2015Article
- Two TIR-like domain containing proteins in a newly emerging zoonotic Staphylococcus aureus strain sequence type 398 are potential virulence factors by impacting on the host innate immune response.Frontiers in microbiology · 2014Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Mammalian toll-like receptor 5 (TLR5) senses flagellin of several bacterial species and has been described to activate the innate immune system. To assess the role of bovine TLR5 (boTLR5) in the cattle system, we cloned and successfully expressed boTLR5 in human embryonic kidney (HEK) 293 cells, as indicated by quantitative PCR and confocal microscopy. However, in contrast to huTLR5-transfected cells, exposure of boTLR5-transfected cells to flagellin neither activated nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) nor CXCL8 production. Subsequent comparison of the flagellin response induced in human and bovine primary macrophages revealed that flagellin did not lead to phosphorylation of major signalling molecules. Furthermore, the CXCL8 and TNFα response of primary bovine macrophages stimulated with flagellin was very low compared to that observed in human primary macrophages. Our results indicate that cattle express a functional TLR5 albeit with different flagellin sensing qualities compared to human TLR5. However, boTLR5 seemed to play a different role in the bovine system compared to the human system in recognizing flagellin, and other potentially intracellular expressed receptors may play a more important role in the bovine system to detect flagellin.
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