ArticleThe Lancet. Respiratory medicine2013
Tropism and innate host responses of a novel avian influenza A H7N9 virus: an analysis of ex-vivo and in-vitro cultures of the human respiratory tract.
Article in The Lancet. Respiratory medicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers, 1 of them a synthesis that pooled it.
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63 citing papers in PubMed, 1 synthesis or guideline pooled it, 100 citations in OpenAlex.
- Serological Evidence of Human Infection With Avian Influenza A(H7N9) Virus: A Systematic Review and Meta-analysis.The Journal of infectious diseases · 2022Pooled it
- Assessment of influenza virus and coronavirus tropism, replication competence and disease severity inThe Journal of general virology · 2026Article
- Interactions between zoonotic pathogens and infectious disease spread: Why understanding mechanisms and modelling matters more than ever.Biosafety and health · 2025Review
- Evaluation of pandemic potential of the genotype 4 (G4) swine influenza virus usingThe Journal of general virology · 2025Article
- The Alarming Situation of Highly Pathogenic Avian Influenza Viruses in 2019-2023.Global medical genetics · 2024Review
- Epistasis mediates the evolution of the receptor binding mode in recent human H3N2 hemagglutinin.Nature communications · 2024Article
- Utility of HumanJournal of virology · 2023Article
- ZBP1/DAI-Dependent Cell Death Pathways in Influenza A Virus Immunity and Pathogenesis.Current topics in microbiology and immunology · 2023Article
- 3DEmerging microbes & infections · 2022Article
- Egg-adaptive mutations of human influenza H3N2 virus are contingent on natural evolution.PLoS pathogens · 2022Article
- Article
- SARS-CoV-2 replicates in respiratory ex vivo organ cultures of domestic ruminant species.Veterinary microbiology · 2021Article
- SARS-CoV-2 receptor ACE2 is co-expressed with genes related to transmembrane serine proteases, viral entry, immunity and cellular stress.Scientific reports · 2020Article
- Assessment of SARS-CoV-2 replication in the context of other respiratory viruses.The Lancet. Respiratory medicine · 2020Article
- Characterizing Emerging Canine H3 Influenza Viruses.PLoS pathogens · 2020Article
- Novel Avian Influenza A Virus Infections of Humans.Infectious disease clinics of North America · 2019Review
- Risk Assessment of the Tropism and Pathogenesis of the Highly Pathogenic Avian Influenza A/H7N9 Virus Using Ex Vivo and In Vitro Cultures of Human Respiratory Tract.The Journal of infectious diseases · 2019Article
- Will China's H7N9 Control Strategy Continue to Be Effective?Open forum infectious diseases · 2019Article
- In Vivo Characterization of Avian Influenza A (H5N1) and (H7N9) Viruses Isolated from Canadian Travelers.Viruses · 2019Article
- Spatiotemporal Variation and Hotspot Detection of the Avian Influenza A(H7N9) Virus in China, 2013⁻2017.International journal of environmental research and public health · 2019Article
3 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors at 1 institution in 2 countries.
Funding
Abstract
backgroundSince March, 2013, an avian-origin influenza A H7N9 virus has caused severe pneumonia in China. The aim of this study was to investigate the pathogenesis of this new virus in human beings.
methodsWe obtained ex-vivo cultures of the human bronchus, lung, nasopharynx, and tonsil and in-vitro cultures of primary human alveolar epithelial cells and peripheral blood monocyte-derived macrophages. We compared virus tropism and induction of proinflammatory cytokine responses of two human influenza A H7N9 virus isolates, A/Shanghai/1/2013 and A/Shanghai/2/2013; a highly pathogenic avian influenza H5N1 virus; the highly pathogenic avian influenza H7N7 virus that infected human beings in the Netherlands in 2003; the 2009 pandemic influenza H1N1 virus, and a low pathogenic duck H7N9 virus that was genetically different to the human disease causing A H7N9 viruses.
findingsBoth human H7N9 viruses replicated efficiently in human bronchus and lung ex-vivo cultures, whereas duck/H7N9 virus failed to replicate in either. Both human A H7N9 viruses infected both ciliated and non-ciliated human bronchial epithelial cells and replicated to higher titres than did H5N1 (p<0.0001 to 0.0046) and A/Shanghai/1/2013 replicated to higher titres than did H7N7 (p=0.0002-0.01). Both human A H7N9 viruses predominantly infected type II alveolar epithelial cells and alveolar macrophages in the human lung and replicated to higher titres than did H5N1 (p<0.0001 to 0.0078); A/Shanghai/1/2013 replicated to higher titres than did H1N1 (p=0.0052-0.05) and H7N7 (p=0.0031-0.0151). Human H7N9 viruses were less potent inducers of proinflammatory cytokines compared with H5N1 virus.
interpretationCollectively, the results suggest that the novel H7N9 viruses are better adapted to infect and replicate in the human conducting and lower airways than are other avian influenza viruses, including H5N1, and pose an important pandemic threat.
fundingArea of Excellence Scheme of the University Grants Committee (AoE/M-12/96), Hong Kong Special Administrative Region.
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