Evidence map›Paper›PMID 24461010›Full record

ArticleBiophysical journal2014

Differentiation of Col I and Col III isoforms in stromal models of ovarian cancer by analysis of second harmonic generation polarization and emission directionality.

Karissa Tilbury, Chi-Hsiang Lien, Shean-Jen Chen, Paul J Campagnola

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.

0numbers the graph read from it
0cells of the map it votes in
38citing papers in PubMed
7.1field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

38 citing papers in PubMed, 73 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Karissa TilburyDepartment of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin.
Chi-Hsiang LienDepartment of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin; Department of Engineering Science, National Cheng Kung University, Tainan, Taiwan.
Shean-Jen ChenDepartment of Engineering Science, National Cheng Kung University, Tainan, Taiwan.
Paul J CampagnolaDepartment of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin; Medical Physics Department, University of Wisconsin-Madison, Madison, Wisconsin. Electronic address: pcampagnola@wisc.edu.
National Cheng Kung University · TWUniversity of Wisconsin–Madison · US

Funding

U.W. RADIOLOGICAL SCIENCES TRAINING PROGRAMT32CA009206 · NCI · UNIVERSITY OF WISCONSIN-MADISON · PI Bryan Patrick Bednarz · 1985 to 2026
$12.1M
Development of The Prairie Technologies MPLSM to image cancer models in vivoR01CA136590 · NCI · UNIVERSITY OF WISCONSIN-MADISON · PI CAMPAGNOLA, PAUL J, KEELY, PATRICIA J · 2009 to 2013
$2.4M
NCI NIH HHS 5T32CA009206-34NCI NIH HHS R01 CA136590NCI NIH HHS R01 CA136590-01A1NCI NIH HHS T32 CA009206
6 · The paper itself

Abstract

A profound remodeling of the extracellular matrix occurs in many epithelial cancers. In ovarian cancer, the minor collagen isoform of Col III becomes upregulated in invasive disease. Here we use second harmonic generation (SHG) imaging microscopy to probe structural differences in fibrillar models of the ovarian stroma comprised of mixtures of Col I and III. The SHG intensity and forward-backward ratios decrease with increasing Col III content, consistent with decreased phasematching due to more randomized structures. We further probe the net collagen α-helix pitch angle within the gel mixtures using what is believed to be a new pixel-based polarization-resolved approach that combines and extends previous analyses. The extracted pitch angles are consistent with those of peptide models and the method has sufficient sensitivity to differentiate Col I from the Col I/Col III mixtures. We further developed the pixel-based approach to extract the SHG signal polarization anisotropy from the same polarization-resolved image matrix. Using this approach, we found that increased Col III results in decreased alignment of the dipole moments within the focal volume. Collectively, the SHG measurements and analysis all indicate that incorporation of Col III results in decreased organization across several levels of collagen organization. Furthermore, the findings suggest that the collagen isoforms comingle within the same fibrils, in good agreement with ultrastructural data. The pixel-based polarization analyses (both excitation and emission) afford determination of structural properties without the previous requirement of having well-aligned fibers, and the approaches should be generally applicable in tissue.

Indexed as

AnimalsAnisotropyCollagen Type ICollagen Type IIIFemaleHumansImage Processing, Computer-AssistedMicroscopyOvarian NeoplasmsProtein IsoformsRatsStromal CellsCollagen Type ICollagen Type IIIProtein Isoforms

Identifiers

PMID24461010
PMCPMC3907237
OpenAlexW2092629410

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.