ArticleBiophysical journal2014
Quantitative analysis of ezrin turnover dynamics in the actin cortex.
Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 66 citations in OpenAlex.
- The actomyosin cortex controls t-tubule remodeling in skeletal muscle.Science advances · 2026Article
- On the mechanism of ezrin activation.Biophysical journal · 2026Article
- The unphosphorylated, closed form of ezrin binds to RNA to maintain a metastatic phenotype in osteosarcoma cells.Science signaling · 2026Article
- The biomechanical signature of tumor invasion.Genes & diseases · 2026Review
- Adherent cells sustain membrane tension gradients independently of migration.Nature communications · 2025Article
- On the Mechanism of Ezrin Activation.bioRxiv : the preprint server for biology · 2025Article
- Ezrin drives adaptation of monocytes to the inflamed lung microenvironment.Cell death & disease · 2024Article
- Actin-bundling protein L-plastin promotes megakaryocyte rigidity and dampens proplatelet formation.Haematologica · 2024Article
- A bipartite NLS motif mediates the nuclear import ofFrontiers in cell and developmental biology · 2024Article
- Modeling the dynamics of actin and myosin during bleb stabilization.bioRxiv : the preprint server for biology · 2023Article
- Article
- Branched actin cortices reconstituted in vesicles sense membrane curvature.Biophysical journal · 2023Article
- Scratching beyond the surface - minimal actin assemblies as tools to elucidate mechanical reinforcement and shape change.Emerging topics in life sciences · 2022Article
- A reduced 1D stochastic model of bleb-driven cell migration.Biophysical journal · 2022Article
- Re-evaluating the actin-dependence of spectraplakin functions during axon growth and maintenance.Developmental neurobiology · 2022Article
- A biophysical perspective of the regulatory mechanisms of ezrin/radixin/moesin proteins.Biophysical reviews · 2022Review
- Influence of membrane-cortex linkers on the extrusion of membrane tubes.Biophysical journal · 2021Article
- Actin-Membrane Release Initiates Cell Protrusions.Developmental cell · 2020Article
- C-terminal phosphorylation modulates ERM-1 localization and dynamics to control cortical actin organization and support lumen formation duringDevelopment (Cambridge, England) · 2020Article
- Membrane-proximal F-actin restricts local membrane protrusions and directs cell migration.Science (New York, N.Y.) · 2020Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Proteins of the ERM family (ezrin, moesin, radixin) play a fundamental role in tethering the membrane to the cellular actin cortex as well as regulating cortical organization and mechanics. Overexpression of dominant inactive forms of ezrin leads to fragilization of the membrane-cortex link and depletion of moesin results in softer cortices that disrupt spindle orientation during cytokinesis. Therefore, the kinetics of association of ERM proteins with the cortex likely influence the timescale of cortical signaling events and the dynamics of membrane interfacing to the cortex. However, little is known about ERM protein turnover at the membrane-cortex interface. Here, we examined cortical ezrin dynamics using fluorescence recovery after photobleaching experiments and single-molecule imaging. Using multiexponential fitting of fluorescence recovery curves, we showed that ezrin turnover resulted from three molecular mechanisms acting on very different timescales. The fastest turnover process was due to association/dissociation from the F-actin cortex, suggesting that ezrin acts as a link that leads to low friction between the membrane and the cortex. The second turnover process resulted from association/dissociation of ezrin from the membrane and the slowest turnover process resulted from the slow diffusion of ezrin in the plane of the membrane. In summary, ezrin-mediated membrane-cortex tethering resulted from long-lived interactions with the membrane via the FERM domain coupled with shorter-lived interactions with the cortex. The slow diffusion of membranous ezrin and its interaction partners relative to the cortex signified that signals emanating from membrane-associated ezrin may locally act to modulate cortical organization and contractility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.