ArticleTissue engineering. Part C, Methods2014
Patterning expression of regenerative growth factors using high intensity focused ultrasound.
Article in Tissue engineering. Part C, Methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Overview of Therapeutic Ultrasound Applications and Safety Considerations: 2024 Update.Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine · 2025Review
- Surgical or Nonsurgical Facial Rejuvenation: The Patients' Choice.Plastic and reconstructive surgery. Global open · 2023Article
- Local delivery of bone morphogenetic protein-2 from near infrared-responsive hydrogels for bone tissue regeneration.Biomaterials · 2020Article
- Using Remote Fields for Complex Tissue Engineering.Trends in biotechnology · 2020Review
- Using Acoustic Fields to Fabricate ECM-Based Biomaterials for Regenerative Medicine Applications.Recent progress in materials · 2020Article
- Leveraging the Imaging Transmit Pulse to Manipulate Phase-Change Nanodroplets for Contrast-Enhanced Ultrasound.IEEE transactions on ultrasonics, ferroelectrics, and frequency control · 2019Article
- Spatiotemporally-controlled transgene expression in hydroxyapatite-fibrin composite scaffolds using high intensity focused ultrasound.Biomaterials · 2019Article
- Effect of low-intensity pulsed ultrasound on osteogenic human mesenchymal stem cells commitment in a new bone scaffold.Journal of applied biomaterials & functional materials · 2017Article
- Use of Hydroxyapatite Doping to Enhance Responsiveness of Heat-Inducible Gene Switches to Focused Ultrasound.Ultrasound in medicine & biology · 2016Article
- Spatial regulation of controlled bioactive factor delivery for bone tissue engineering.Advanced drug delivery reviews · 2015Review
- Clinical Application of Mesenchymal Stem Cells and Novel Supportive Therapies for Oral Bone Regeneration.BioMed research international · 2015Review
- Temporal and spatial patterning of transgene expression by near-infrared irradiation.Biomaterials · 2014Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 3 countries.
Funding
Abstract
Temporal and spatial control of growth factor gradients is critical for tissue patterning and differentiation. Reinitiation of this developmental program is also required for regeneration of tissues during wound healing and tissue regeneration. Devising methods for reconstituting growth factor gradients remains a central challenge in regenerative medicine. In the current study we develop a novel gene therapy approach for temporal and spatial control of two important growth factors in bone regeneration, vascular endothelial growth factor, and bone morphogenetic protein 2, which involves application of high intensity focused ultrasound to cells engineered with a heat-activated- and ligand-inducible gene switch. Induction of transgene expression was tightly localized within cell-scaffold constructs to subvolumes of ∼30 mm³, and the amplitude and projected area of transgene expression was tuned by the intensity and duration of ultrasound exposure. Conditions for ultrasound-activated transgene expression resulted in minimal cytotoxicity and scaffold damage. Localized regions of growth factor expression also established gradients in signaling activity, suggesting that patterns of growth factor expression generated by this method will have utility in basic and applied studies on tissue development and regeneration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.