ArticleNucleic acids research2014
Suppression of intragenic transcription requires the MOT1 and NC2 regulators of TATA-binding protein.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 21 citations in OpenAlex.
- An essential gene screening identifies yeast Mot1 as a suppressor of R-loops and genome instability.PLoS genetics · 2026Article
- High-resolution binding data of TFIID and cofactors show promoter-specific differences in vivo.Research square · 2026Article
- High-resolution binding data of TFIID and cofactors show promoter-specific differencesbioRxiv : the preprint server for biology · 2025Article
- Integrating quantitative proteomics with accurate genome profiling of transcription factors by greenCUT&RUN.Nucleic acids research · 2021Article
- Integrated genomic analysis reveals key features of long undecoded transcript isoform-based gene repression.Molecular cell · 2021Article
- Tunable Transcriptional Interference at the Endogenous Alcohol Dehydrogenase Gene Locus inG3 (Bethesda, Md.) · 2020Article
- Structure and mechanism of the RNA polymerase II transcription machinery.Genes & development · 2020Review
- Identification of Two Distinct Classes of the Human INO80 Complex Genome-Wide.G3 (Bethesda, Md.) · 2018Article
- A regulatory circuit of two lncRNAs and a master regulator directs cell fate in yeast.Nature communications · 2018Article
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- Article
- Transcription of the mating-type-regulated lncRNA IRT1 is governed by TORC1 and PKA.Current genetics · 2017Article
- Defects in the NC2 repressor affect both canonical and non-coding RNA polymerase II transcription initiation in yeast.BMC genomics · 2016Article
- Article
- Regulation of anti-sense transcription by Mot1p and NC2 via removal of TATA-binding protein (TBP) from the 3'-end of genes.Nucleic acids research · 2015Article
- Meeting report: 11th EMBL conference on transcription and chromatin - August 23-26, 2014 - Heidelberg, Germany.Epigenetics · 2014Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Chromatin structure in transcribed regions poses a barrier for intragenic transcription. In a comprehensive study of the yeast chromatin remodelers and the Mot1p-NC2 regulators of TATA-binding protein (TBP), we detected synthetic genetic interactions indicative of suppression of intragenic transcription. Conditional depletion of Mot1p or NC2 in absence of the ISW1 remodeler, but not in the absence of other chromatin remodelers, activated the cryptic FLO8 promoter. Likewise, conditional depletion of Mot1p or NC2 in deletion backgrounds of the H3K36 methyltransferase Set2p or the Asf1p-Rtt106p histone H3-H4 chaperones, important factors involved in maintaining a repressive chromatin environment, resulted in increased intragenic FLO8 transcripts. Activity of the cryptic FLO8 promoter is associated with reduced H3 levels, increased TBP binding and tri-methylation of H3K4 and is independent of Spt-Ada-Gcn5-acetyltransferase function. These data reveal cooperation of negative regulation of TBP with specific chromatin regulators to inhibit intragenic transcription.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.