ArticleJournal of visualized experiments : JoVE2014
Determining the ice-binding planes of antifreeze proteins by fluorescence-based ice plane affinity.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 47 citations in OpenAlex.
- Winter-active spiders (Clubiona) have a hyperactive antifreeze protein with a unique beta-solenoid fold.The FEBS journal · 2026Article
- How to measure the activity of antifreeze proteins?Biophysical reviews · 2025Review
- Identification of ice-binding proteins from Raphanus sativus and application in frozen dough.NPJ science of food · 2025Article
- Molecular basis for inhibition of methane clathrate growth by a deep subsurface bacterial protein.PNAS nexus · 2023Article
- Adsorption of ice-binding proteins onto whole ice crystal surfaces does not necessarily confer a high thermal hysteresis activity.Scientific reports · 2022Article
- Discovery of Hyperactive Antifreeze Protein from Phylogenetically Distant Beetles Questions Its Evolutionary Origin.International journal of molecular sciences · 2021Article
- Characterization of microbial antifreeze protein with intermediate activity suggests that a bound-water network is essential for hyperactivity.Scientific reports · 2021Article
- Antifreeze Proteins and Their Practical Utilization in Industry, Medicine, and Agriculture.Biomolecules · 2020Review
- An Ice-Binding Protein from an Antarctic Ascomycete Is Fine-Tuned to Bind to Specific Water Molecules Located in the Ice Prism Planes.Biomolecules · 2020Article
- Fish-Derived Antifreeze Proteins and Antifreeze Glycoprotein Exhibit a Different Ice-Binding Property with Increasing Concentration.Biomolecules · 2020Article
- Peptidic Antifreeze Materials: Prospects and Challenges.International journal of molecular sciences · 2019Review
- Calcium-Binding Generates the Semi-Clathrate Waters on a Type II Antifreeze Protein to Adsorb onto an Ice Crystal Surface.Biomolecules · 2019Article
- Ice recrystallization is strongly inhibited when antifreeze proteins bind to multiple ice planes.Scientific reports · 2019Article
- Polypentagonal ice-like water networks emerge solely in an activity-improved variant of ice-binding protein.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- From ice-binding proteins to bio-inspired antifreeze materials.Soft matter · 2017Review
- The interfacial structure of water droplets in a hydrophobic liquid.Nature communications · 2017Article
- Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification.Journal of visualized experiments : JoVE · 2017Article
- Concentration-dependent oligomerization of an alpha-helical antifreeze polypeptide makes it hyperactive.Scientific reports · 2017Article
- Microfluidic Cold-Finger Device for the Investigation of Ice-Binding Proteins.Biophysical journal · 2016Article
- Putting life on ice: bacteria that bind to frozen water.Journal of the Royal Society, Interface · 2016Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antifreeze proteins (AFPs) are expressed in a variety of cold-hardy organisms to prevent or slow internal ice growth. AFPs bind to specific planes of ice through their ice-binding surfaces. Fluorescence-based ice plane affinity (FIPA) analysis is a modified technique used to determine the ice planes to which the AFPs bind. FIPA is based on the original ice-etching method for determining AFP-bound ice-planes. It produces clearer images in a shortened experimental time. In FIPA analysis, AFPs are fluorescently labeled with a chimeric tag or a covalent dye then slowly incorporated into a macroscopic single ice crystal, which has been preformed into a hemisphere and oriented to determine the a- and c-axes. The AFP-bound ice hemisphere is imaged under UV light to visualize AFP-bound planes using filters to block out nonspecific light. Fluorescent labeling of the AFPs allows real-time monitoring of AFP adsorption into ice. The labels have been found not to influence the planes to which AFPs bind. FIPA analysis also introduces the option to bind more than one differently tagged AFP on the same single ice crystal to help differentiate their binding planes. These applications of FIPA are helping to advance our understanding of how AFPs bind to ice to halt its growth and why many AFP-producing organisms express multiple AFP isoforms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.