ArticleJournal of visualized experiments : JoVE2014
Dissection of the transversus abdominis muscle for whole-mount neuromuscular junction analysis.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Maternal-Fetal Administration of Risdiplam Partially Rescues the SMNΔ7 Mouse Model of Spinal Muscular Atrophy.Annals of neurology · 2026Article
- An Optimized Ex Vivo Protocol for Quantitative Electrophysiological Assessment of Neuromuscular Junctions and Skeletal Muscle Function Using the Aurora System.Bio-protocol · 2025Article
- Improved gene therapy for spinal muscular atrophy in mice using codon-optimized hSMN1 transgene and hSMN1 gene-derived promotor.EMBO molecular medicine · 2024Article
- Article
- Dissection, in vivo imaging and analysis of the mouse epitrochleoanconeus muscle.Journal of anatomy · 2022Article
- Suppression of the necroptotic cell death pathways improves survival inFrontiers in cellular neuroscience · 2022Article
- NMJ-Analyser identifies subtle early changes in mouse models of neuromuscular disease.Scientific reports · 2021Article
- Morphological variability is greater at developing than mature mouse neuromuscular junctions.Journal of anatomy · 2020Article
- Synaptic withdrawal following nerve injury is influenced by postnatal maturity, muscle-specific properties, and the presence of underlying pathology in mice.Journal of anatomy · 2020Article
- Developmental demands contribute to early neuromuscular degeneration in CMT2D mice.Cell death & disease · 2020Article
- Neuropilin 1 sequestration by neuropathogenic mutant glycyl-tRNA synthetase is permissive to vascular homeostasis.Scientific reports · 2017Article
- NMJ-morph reveals principal components of synaptic morphology influencing structure-function relationships at the neuromuscular junction.Open biology · 2016Article
- Effect of genetic background on the phenotype of the Smn2B/- mouse model of spinal muscular atrophy.Human molecular genetics · 2016Article
- Morphological analysis of neuromuscular junction development and degeneration in rodent lumbrical muscles.Journal of neuroscience methods · 2014Article
- The Smn-independent beneficial effects of trichostatin A on an intermediate mouse model of spinal muscular atrophy.PloS one · 2014Article
- Article
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3 authors.
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Abstract
Analysis of neuromuscular junction morphology can give important insight into the physiological status of a given motor neuron. Analysis of thin flat muscles can offer significant advantage over traditionally used thicker muscles, such as those from the hind limb (e.g. gastrocnemius). Thin muscles allow for comprehensive overview of the entire innervation pattern for a given muscle, which in turn permits identification of selectively vulnerable pools of motor neurons. These muscles also allow analysis of parameters such as motor unit size, axonal branching, and terminal/nodal sprouting. A common obstacle in using such muscles is gaining the technical expertise to dissect them. In this video, we detail the protocol for dissecting the transversus abdominis (TVA) muscle from young mice and performing immunofluorescence to visualize axons and neuromuscular junctions (NMJs). We demonstrate that this technique gives a complete overview of the innervation pattern of the TVA muscle and can be used to investigate NMJ pathology in a mouse model of the childhood motor neuron disease, spinal muscular atrophy.
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