ArticleGenes to cells : devoted to molecular & cellular mechanisms2014
Derivation of human differential photoreceptor cells from adult human dermal fibroblasts by defined combinations of CRX, RAX, OTX2 and NEUROD.
Article in Genes to cells : devoted to molecular & cellular mechanisms, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 31 citations in OpenAlex.
- Direct reprogramming of Müller glia into photoreceptors via multiple transcription factors and small molecules: molecular mechanisms and transcriptomic analysis.In vitro cellular & developmental biology. Animal · 2026Article
- Linking Order of RAX and OTX2 Following CRX in Polycistronic Vectors Can Partially Determine Efficacy of Direct Conversion to Photoreceptor-like Cells In Vitro.Cell biochemistry and biophysics · 2026Article
- Evaluation of mesenchymal stem cells as anFrontiers in cell and developmental biology · 2024Review
- [Optogenetics and cell replacement in retinology : Regenerative ophthalmology-What we can do!]Die Ophthalmologie · 2022Review
- One-step induction of photoreceptor-like cells from human iPSCs by delivering transcription factors.iScience · 2022Article
- Evaluation of photoreceptor-directed fibroblasts derived from retinitis pigmentosa patients with defects in the EYS gene: a possible cost-effective cellular model for mechanism-oriented drug.Stem cell research & therapy · 2022Article
- Neuronal Reprogramming for Tissue Repair and Neuroregeneration.International journal of molecular sciences · 2020Review
- Direct cell-fate conversion of somatic cells: Toward regenerative medicine and industries.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2020Review
- Using transcription factors for direct reprogramming of neuronsWorld journal of stem cells · 2019Review
- Otx2 enhances transdifferentiation of Müller cells-derived retinal stem cells into photoreceptor-like cells.Journal of cellular and molecular medicine · 2019Article
- Overexpression of MiR-183/96/182 Triggers Retina-Like Fate in Human Bone Marrow-Derived Mesenchymal Stem Cells (hBMSCs) in Culture.Journal of ophthalmology · 2019Article
- The manner of decay of genetically defective EYS gene transcripts in photoreceptor-directed fibroblasts derived from retinitis pigmentosa patients depends on the type of mutation.Stem cell research & therapy · 2018Article
- In vitro transdifferentiation of human peripheral blood mononuclear cells to photoreceptor-like cells.Biology open · 2016Article
- Advances in understanding the molecular basis of the first steps in color vision.Progress in retinal and eye research · 2015Review
- Zebrafish Lbh-like Is Required for Otx2-mediated Photoreceptor Differentiation.International journal of biological sciences · 2015Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Redirecting differentiation of somatic cells by over-expression of transcription factors is a promising approach for regenerative medicine, elucidation of pathogenesis and development of new therapies. We have previously defined a transcription factor combination, that is, CRX, RAX and NEUROD, that can generate photosensitive photoreceptor cells from human iris cells. Here, we show that human dermal fibroblasts are differentiated to photoreceptor cells by the same transcription factor combination as human iris cells. Transduction of a combination of the CRX, RAX and NEUROD genes up-regulated expression of the photoreceptor-specific genes, recoverin, blue opsin and PDE6C, in all three strains of human dermal fibroblasts that were tested. Additional OTX2 gene transduction increased up-regulation of the photoreceptor-specific genes blue opsin, recoverin, S-antigen, CNGB3 and PDE6C. Global gene expression data by microarray analysis further showed that photoreceptor-related functional genes were significantly increased in induced photoreceptor cells. Functional analysis, that is, patch-clamp recordings, clearly revealed that induced photoreceptor cells from fibroblasts responded to light. Both the NRL gene and the NR2E3 gene were endogenously up-regulated in induced photoreceptor cells, implying that exogenous CRX, RAX, OTX2 and NEUROD, but not NRL, are sufficient to generate rod photoreceptor cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.