ArticlePloS one2014
A co-culture model of the developing small intestine offers new insight in the early immunomodulation of enterocytes and macrophages by Lactobacillus spp. through STAT1 and NF-kB p65 translocation.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 47 citations in OpenAlex.
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- Assessing immunogenicity of CRISPR-NCas9 engineered strain against porcine epidemic diarrhea virus.Applied microbiology and biotechnology · 2024Article
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- Characterization of exosomes derived from IPEC-J2 treated with probioticFrontiers in immunology · 2022Article
- Effect of the Microbiome on Intestinal Innate Immune Development in Early Life and the Potential Strategy of Early Intervention.Frontiers in immunology · 2022Review
- Coculture of primary human colon monolayer with human gut bacteria.Nature protocols · 2021Article
- Probiotics: a Promising Generation of Heavy Metal Detoxification.Biological trace element research · 2021Review
- Isolation, characterisation and phagocytic function of human macrophages from human peripheral blood.Molecular biology reports · 2020Article
- The Lipocalin2 Gene is Regulated in Mammary Epithelial Cells by NFκB and C/EBP In Response to Mycoplasma.Scientific reports · 2020Article
- Probiotic mediated NF-κB regulation for prospective management of type 2 diabetes.Molecular biology reports · 2020Review
- Effect of Lactobacillus spp. on adhesion, invasion, and translocation of Campylobacter jejuni in chicken and pig small-intestinal epithelial cell lines.BMC veterinary research · 2020Article
- Adhesion mechanisms mediated by probiotics and prebiotics and their potential impact on human health.Applied microbiology and biotechnology · 2019Review
- Nonsteroidal Anti-Inflammatory Drug-Induced Leaky Gut Modeled Using Polarized Monolayers of Primary Human Intestinal Epithelial Cells.ACS infectious diseases · 2018Article
- Comparison of conventional and advanced in vitro models in the toxicity testing of nanoparticles.Artificial cells, nanomedicine, and biotechnology · 2018Review
- IntestinalFrontiers in physiology · 2018Review
- A primary human macrophage-enteroid co-culture model to investigate mucosal gut physiology and host-pathogen interactions.Scientific reports · 2017Article
- A novel technique to determine the cell type specific response within an in vitro co-culture model via multi-colour flow cytometry.Scientific reports · 2017Article
- Single-cell analysis reveals IGF-1 potentiation of inhibition of the TGF-β/Smad pathway of fibrosis in human keratocytes in vitro.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The early establishment of a complete microbiome has been shown to play an integral part in the development and maintenance of an intact intestine and its immune system, although much remains unknown about the specific mechanisms of immune modulation in newborns. In our study we show in a co-culture model of the undeveloped small intestine that members of Lactobacillus spp. influence STAT1 and NF-kB p65 nuclear translocation in both intestinal epithelial cells as well as underlying macrophages. Moreover, by using imaging flow cytometry we were able to monitor each individual cell and create a framework of the percentage of cells in which translocation occurred in challenged versus control cell populations. We also observed a significant difference in baseline translocation in intestinal cells when cultured alone versus those in a co-culture model, underpinning the importance of 3D models over monolayer set-ups in epithelial in vitro research. In conclusion, our work offers new insights into the potential routes by which the commensal microbiome primes the early immune system to fight pathogens, and shows how strain-specific these mechanisms really are.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.