ArticlePloS one2014
The RNA chaperone Hfq impacts growth, metabolism and production of virulence factors in Yersinia enterocolitica.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
- Study on the impact of Hfq regulation on spoilage odor formation byFood chemistry: X · 2026Article
- Microgravity-induced constraints on melanin bioproduction: investigating E. coli metabolic responses aboard the international space station.NPJ microgravity · 2026Article
- Structure and dynamics of Proteus vulgaris tryptophan indole-lyase complexes with l-ethionine and l-alanine.Archives of biochemistry and biophysics · 2025Article
- Structural Snapshots ofACS catalysis · 2024Article
- The RNA chaperone Hfq has a multifaceted role inFrontiers in cellular and infection microbiology · 2024Article
- Interplay between the RNA Chaperone Hfq, Small RNAs and Transcriptional Regulator OmpR Modulates Iron Homeostasis in the EnteropathogenInternational journal of molecular sciences · 2023Article
- Role of Hfq in glucose utilization, biofilm formation and quorum sensing system in Bacillus subtilis.Biotechnology letters · 2022Article
- The RNA Chaperone Hfq Participates in Persistence to Multiple Antibiotics in the Fish PathogenMicroorganisms · 2021Article
- The Multiple Regulatory Relationship Between RNA-Chaperone Hfq and the Second Messenger c-di-GMP.Frontiers in microbiology · 2021Review
- Urease Expression in PathogenicFrontiers in microbiology · 2020Article
- The small RNA RssR regulates myo-inositol degradation by Salmonella enterica.Scientific reports · 2018Article
- Reciprocal Regulation of OmpR and Hfq and Their Regulatory Actions on the Vi Polysaccharide Capsular Antigen in Salmonella enterica Serovar Typhi.Current microbiology · 2018Article
- Enteropathogens: Tuning Their Gene Expression for Hassle-Free Survival.Frontiers in microbiology · 2018Review
- Discovering RNA-Based Regulatory Systems forFrontiers in cellular and infection microbiology · 2018Review
- Post-transcriptional regulation of type III secretion in plant and animal pathogens.Current opinion in microbiology · 2017Review
- Yersinia enterocolitica-Specific Infection by Bacteriophages TG1 and ϕR1-RT Is Dependent on Temperature-Regulated Expression of the Phage Host Receptor OmpF.Applied and environmental microbiology · 2016Article
- The RNA Chaperone Hfq Is Essential for Virulence and Modulates the Expression of Four Adhesins in Yersinia enterocolitica.Scientific reports · 2016Article
- Environmental Regulation of Yersinia Pathophysiology.Frontiers in cellular and infection microbiology · 2016Review
- Genome-wide analysis of small RNAs expressed by Yersinia pestis identifies a regulator of the Yop-Ysc type III secretion system.Journal of bacteriology · 2014Article
- Profound impact of Hfq on nutrient acquisition, metabolism and motility in the plant pathogen Agrobacterium tumefaciens.PloS one · 2014Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To adapt to changes in environmental conditions, bacteria regulate their gene expression at the transcriptional but also at the post-transcriptional level, e.g. by small RNAs (sRNAs) which modulate mRNA stability and translation. The conserved RNA chaperone Hfq mediates the interaction of many sRNAs with their target mRNAs, thereby playing a global role in fine-tuning protein production. In this study, we investigated the significance of Hfq for the enteropathogen Yersina enterocolitica serotype O:8. Hfq facilitated optimal growth in complex and minimal media. Our comparative protein analysis of parental and hfq-negative strains suggested that Hfq promotes lipid metabolism and transport, cell redox homeostasis, mRNA translation and ATP synthesis, and negatively affects carbon and nitrogen metabolism, transport of siderophore and peptides and tRNA synthesis. Accordingly, biochemical tests indicated that Hfq represses ornithine decarboxylase activity, indole production and utilization of glucose, mannitol, inositol and 1,2-propanediol. Moreover, Hfq repressed production of the siderophore yersiniabactin and its outer membrane receptor FyuA. In contrast, hfq mutants exhibited reduced urease production. Finally, strains lacking hfq were more susceptible to acidic pH and oxidative stress. Unlike previous reports in other Gram-negative bacteria, Hfq was dispensable for type III secretion encoded by the virulence plasmid. Using a chromosomally encoded FLAG-tagged Hfq, we observed increased production of Hfq-FLAG in late exponential and stationary phases. Overall, Hfq has a profound effect on metabolism, resistance to stress and modulates the production of two virulence factors in Y. enterocolitica, namely urease and yersiniabactin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.