ArticlePloS one2014
The RNA chaperone Hfq is involved in stress tolerance and virulence in uropathogenic Proteus mirabilis.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- Bioinformatic Analyses of the Ataxin-2 Family Since Algae Emphasize Its Small Isoforms, Large Chimerisms, and the Importance of Human Exon 1B as Target of Therapies to Prevent Neurodegeneration.International journal of molecular sciences · 2026Article
- The RNA chaperone Hfq has a multifaceted role inFrontiers in cellular and infection microbiology · 2024Article
- Unveiling the hidden arsenal: new insights intoFrontiers in cellular and infection microbiology · 2024Review
- The QseEF Two-Component System-GlmY Small RNA Regulatory Pathway Controls Swarming in UropathogenicInternational journal of molecular sciences · 2022Article
- The RNA Chaperone Hfq Participates in Persistence to Multiple Antibiotics in the Fish PathogenMicroorganisms · 2021Article
- Crc Regulates Succinate-Mediated Repression of Mineral Phosphate Solubilization inFrontiers in microbiology · 2021Article
- Deacidification by FhlA-dependent hydrogenase is involved in urease activity and urinary stone formation in uropathogenic Proteus mirabilis.Scientific reports · 2020Article
- Alternative Enzyme Protection Assay To Overcome the Drawbacks of the Gentamicin Protection Assay for Measuring Entry and Intracellular Survival of Staphylococci.Infection and immunity · 2019Article
- In silico 'fishing' using known small regulatory RNA (sRNA) candidates as the decoy from Escherichia coli, Salmonella typhi and Salmonella typhimurium manifested 14 novel sRNA candidates in the orthologous region of Proteus mirabilis.Molecular biology reports · 2018Article
- Pathogenesis ofEcoSal Plus · 2018Review
- Article
- An A/U-Rich Enhancer Region Is Required for High-Level Protein Secretion through the HlyA Type I Secretion System.Applied and environmental microbiology · 2018Article
- cAMP receptor protein regulates mouse colonization, motility, fimbria-mediated adhesion, and stress tolerance in uropathogenic Proteus mirabilis.Scientific reports · 2017Article
- Functional Characterization ofFrontiers in microbiology · 2017Article
- The Small RNA GcvB Promotes Mutagenic Break Repair by Opposing the Membrane Stress Response.Journal of bacteriology · 2016Article
- Involvement of polyphosphate kinase in virulence and stress tolerance of uropathogenic Proteus mirabilis.Medical microbiology and immunology · 2016Article
- New aspects of RpoE in uropathogenic Proteus mirabilis.Infection and immunity · 2015Article
- RNA-binding proteins involved in post-transcriptional regulation in bacteria.Frontiers in microbiology · 2015Review
- Overexpression of an outer membrane protein associated with decreased susceptibility to carbapenems in Proteus mirabilis.PloS one · 2015Article
- Cell-based assay to identify inhibitors of the Hfq-sRNA regulatory pathway.Antimicrobial agents and chemotherapy · 2014Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hfq is a bacterial RNA chaperone involved in the riboregulation of diverse genes via small noncoding RNAs. Here, we show that Hfq is critical for the uropathogenic Proteus mirabilis to effectively colonize the bladder and kidneys in a murine urinary tract infection (UTI) model and to establish burned wound infection of the rats. In this regard, we found the hfq mutant induced higher IL-8 and MIF levels of uroepithelial cells and displayed reduced intra-macrophage survival. The loss of hfq affected bacterial abilities to handle H2O2 and osmotic pressures and to grow at 50 °C. Relative to wild-type, the hfq mutant had reduced motility, fewer flagella and less hemolysin expression and was less prone to form biofilm and to adhere to and invade uroepithelial cells. The MR/P fimbrial operon was almost switched to the off phase in the hfq mutant. In addition, we found the hfq mutant exhibited an altered outer membrane profile and had higher RpoE expression, which indicates the hfq mutant may encounter increased envelope stress. With the notion of envelope disturbance in the hfq mutant, we found increased membrane permeability and antibiotic susceptibilities in the hfq mutant. Finally, we showed that Hfq positively regulated the RpoS level and tolerance to H2O2 in the stationary phase seemed largely mediated through the Hfq-dependent RpoS expression. Together, our data indicate that Hfq plays a critical role in P. mirabilis to establish UTIs by modulating stress responses, surface structures and virulence factors. This study suggests Hfq may serve as a scaffold molecule for development of novel anti-P. mirabilis drugs and P. mirabilis hfq mutant is a vaccine candidate for preventing UTIs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.