ArticlePloS one2014
PprA contributes to Deinococcus radiodurans resistance to nalidixic acid, genome maintenance after DNA damage and interacts with deinococcal topoisomerases.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 42 citations in OpenAlex.
- Application of the transposon-associated TnpB system of CRISPR-Cas in bacteria:Frontiers in microbiology · 2025Review
- DdrC, a unique DNA repair factor from D. radiodurans, senses and stabilizes DNA breaks through a novel lesion-recognition mechanism.Nucleic acids research · 2024Article
- The Deinococcus protease PprI senses DNA damage by directly interacting with single-stranded DNA.Nature communications · 2024Article
- DNA damage response and cell cycle regulation in bacteria: a twist around the paradigm.Frontiers in microbiology · 2024Article
- Characterization of the DdrD protein from the extremely radioresistant bacterium Deinococcus radiodurans.Extremophiles : life under extreme conditions · 2021Article
- PprA Protein Inhibits DNA Strand Exchange and ATP Hydrolysis ofFrontiers in cell and developmental biology · 2021Article
- PprI: The Key Protein in Response to DNA Damage inFrontiers in cell and developmental biology · 2020Review
- Conservation and diversity of radiation and oxidative stress resistance mechanisms in Deinococcus species.FEMS microbiology reviews · 2019Review
- Maintenance of multipartite genome system and its functional significance in bacteria.Journal of genetics · 2018Review
- Conservation and diversity of the IrrE/DdrO-controlled radiation response in radiation-resistant Deinococcus bacteria.MicrobiologyOpen · 2017Article
- In vivo and in vitro characterization of DdrC, a DNA damage response protein in Deinococcus radiodurans bacterium.PloS one · 2017Article
- DNA Gyrase of Deinococcus radiodurans is characterized as Type II bacterial topoisomerase and its activity is differentially regulated by PprA in vitro.Extremophiles : life under extreme conditions · 2016Article
- PprM, a Cold Shock Domain-Containing Protein fromFrontiers in microbiology · 2016Article
- Topoisomerase IB of Deinococcus radiodurans resolves guanine quadruplex DNA structures in vitro.Journal of biosciences · 2015Article
- Article
- Expression and mutational analysis of DinB-like protein DR0053 in Deinococcus radiodurans.PloS one · 2015Article
- Article
- Interaction of double-stranded DNA with polymerized PprA protein from Deinococcus radiodurans.Protein science : a publication of the Protein Society · 2014Article
- Dr-FtsA, an actin homologue in Deinococcus radiodurans differentially affects Dr-FtsZ and Ec-FtsZ functions in vitro.PloS one · 2014Article
- Molecular insights into replication initiation in a multipartite genome harboring bacterium Deinococcus radiodurans.The Journal of biological chemistryArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
PprA is known to contribute to Deinococcus radiodurans' remarkable capacity to survive a variety of genotoxic assaults. The molecular bases for PprA's role(s) in the maintenance of the damaged D. radiodurans genome are incompletely understood, but PprA is thought to promote D. radiodurans's capacity for DSB repair. PprA is found in a multiprotein DNA processing complex along with an ATP type DNA ligase, and the D. radiodurans toposiomerase IB (DraTopoIB) as well as other proteins. Here, we show that PprA is a key contributor to D. radiodurans resistance to nalidixic acid (Nal), an inhibitor of topoisomerase II. Growth of wild type D. radiodurans and a pprA mutant were similar in the absence of exogenous genotoxic insults; however, the pprA mutant exhibited marked growth delay and a higher frequency of anucleate cells following treatment with DNA-damaging agents. We show that PprA interacts with both DraTopoIB and the Gyrase A subunit (DraGyrA) in vivo and that purified PprA enhances DraTopoIB catalysed relaxation of supercoiled DNA. Thus, besides promoting DNA repair, our findings suggest that PprA also contributes to preserving the integrity of the D. radiodurans genome following DNA damage by interacting with DNA topoisomerases and by facilitating the actions of DraTopoIB.
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