Evidence map›Paper›PMID 24454803›Full record

ArticlePloS one2014

Comprehensive genetic analysis of early host body reactions to the bioactive and bio-inert porous scaffolds.

Tomo Ehashi, Taro Takemura, Nobutaka Hanagata, Takashi Minowa, Hisatoshi Kobayashi, Kazuhiko Ishihara, Tetsuji Yamaoka

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
2.7field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 21 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Delivery strategies to control inflammatory response: Modulating M1-M2 polarization in tissue engineering applications.Journal of controlled release : official journal of the Controlled Release Society · 2016
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Tomo EhashiDepartment of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Osaka, Japan ; Core Research Evolutional Science and Technology (CREST), Japan Science and Technology Agency, Tokyo, Japan.
Taro TakemuraNanotechnology Innovation Station, National Institute for Materials Science, Ibaraki, Japan.
Nobutaka HanagataNanotechnology Innovation Station, National Institute for Materials Science, Ibaraki, Japan.
Takashi MinowaNanotechnology Innovation Station, National Institute for Materials Science, Ibaraki, Japan.
Hisatoshi KobayashiBiomaterials Center, National Institute for Materials Science, Ibaraki, Japan.
Kazuhiko IshiharaDepartment of Materials Engineering and Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan.
Tetsuji YamaokaDepartment of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Osaka, Japan ; Core Research Evolutional Science and Technology (CREST), Japan Science and Technology Agency, Tokyo, Japan.
National Institute for Materials Science · JPJapan Science and Technology Agency · JPNational Cerebral and Cardiovascular Center · JPThe University of Tokyo · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

To design scaffolds for tissue regeneration, details of the host body reaction to the scaffolds must be studied. Host body reactions have been investigated mainly by immunohistological observations for a long time. Despite of recent dramatic development in genetic analysis technologies, genetically comprehensive changes in host body reactions are hardly studied. There is no information about host body reactions that can predict successful tissue regeneration in the future. In the present study, porous polyethylene scaffolds were coated with bioactive collagen or bio-inert poly(2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate) (PMB) and were implanted subcutaneously and compared the host body reaction to those substrates by normalizing the result using control non-coat polyethylene scaffold. The comprehensive analyses of early host body reactions to the scaffolds were carried out using a DNA microarray assay. Within numerous genes which were expressed differently among these scaffolds, particular genes related to inflammation, wound healing, and angiogenesis were focused upon. Interleukin (IL)-1β and IL-10 are important cytokines in tissue responses to biomaterials because IL-1β promotes both inflammation and wound healing and IL-10 suppresses both of them. IL-1β was up-regulated in the collagen-coated scaffold. Collagen-specifically up-regulated genes contained both M1- and M2-macrophage-related genes. Marked vessel formation in the collagen-coated scaffold was occurred in accordance with the up-regulation of many angiogenesis-inducible factors. The DNA microarray assay provided global information regarding the host body reaction. Interestingly, several up-regulated genes were detected even on the very bio-inert PMB-coated surfaces and those genes include inflammation-suppressive and wound healing-suppressive IL-10, suggesting that not only active tissue response but also the inert response may relates to these genetic regulations.

Indexed as

AnimalsBiocompatible MaterialsCell MovementCollagenGene Expression RegulationMacrophagesMaleMaterials TestingMethacrylatesMiceMice, Inbred C57BLPhosphorylcholinePolyethylenePorositySurface PropertiesTime FactorsBiocompatible MaterialsCollagenMethacrylatesPhosphorylcholinepoly(2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate)Polyethylene

Identifiers

PMID24454803
PMCPMC3891765
OpenAlexW2076540310

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.