ArticlePloS one2014
Transposon defense by endo-siRNAs, piRNAs and somatic pilRNAs in Drosophila: contributions of Loqs-PD and R2D2.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 29 citations in OpenAlex.
- Limited impact of the siRNA pathway on transposable element expression in Aedes aegypti.BMC biology · 2025Article
- A guide to the biogenesis and functions of endogenous small non-coding RNAs in animals.Nature reviews. Molecular cell biology · 2025Review
- Review
- Piwi maintains homeostasis in the Drosophila adult intestine.Stem cell reports · 2023Article
- Three alternative splicing variants ofRNA biology · 2023Article
- Transposable element landscapes in aging Drosophila.PLoS genetics · 2022Article
- Specific PIWI-Interacting RNAs and Related Small Noncoding RNAs Are Associated With Ovarian Aging in Ames Dwarf (df/df) Mice.The journals of gerontology. Series A, Biological sciences and medical sciences · 2021Article
- A mosquito small RNA genomics resource reveals dynamic evolution and host responses to viruses and transposons.Genome research · 2021Article
- Transposable elements, contributors in the evolution of organisms (from an arms race to a source of raw materials).Heliyon · 2021Review
- Review
- Viral infection impacts transposable element transcript amounts inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Dual-layer transposon repression in heads ofRNA (New York, N.Y.) · 2018Article
- Systemic signalling and local effectors in developmental stability, body symmetry, and size.Cell stress · 2018Review
- Loquacious-PD removes phosphate inhibition of Dicer-2 processing of hairpin RNAs into siRNAs.Biochemical and biophysical research communications · 2018Article
- Molecular basis for asymmetry sensing of siRNAs by the Drosophila Loqs-PD/Dcr-2 complex in RNA interference.Nucleic acids research · 2017Article
- Overexpression and purification of Dicer and accessory proteins for biochemical and structural studies.Methods (San Diego, Calif.) · 2017Article
- Expression of the Retrotransposon Helena Reveals a Complex Pattern of TE Deregulation in Drosophila Hybrids.PloS one · 2016Article
- Changes of Osvaldo expression patterns in germline of male hybrids between the species Drosophila buzzatii and Drosophila koepferae.Molecular genetics and genomics : MGG · 2015Article
- Drosophila dicer-2 cleavage is mediated by helicase- and dsRNA termini-dependent states that are modulated by Loquacious-PD.Molecular cell · 2015Article
- Host control of insect endogenous retroviruses: small RNA silencing and immune response.Viruses · 2014Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transposable elements are a serious threat for genome integrity and their control via small RNA mediated silencing pathways is an ancient strategy. The fruit fly Drosophila melanogaster has two silencing activities that target transposons: endogenous siRNAs (esiRNAs or endo-siRNAs) and Piwi-interacting small RNAs (piRNAs). The biogenesis of endo-siRNAs involves the Dicer-2 co-factors Loqs-PD, which acts predominantly during processing of dsRNA by Dcr-2, and R2D2, which primarily helps to direct siRNAs into the RNA interference effector Ago2. Nonetheless, loss of either protein is not sufficient to produce a phenotype comparable with a dcr-2 mutation. We provide further deep sequencing evidence supporting the notion that R2D2 and Loqs-PD have partially overlapping function. Certain transposons display a preference for either dsRBD-protein during production or loading; this appeared to correlate neither with overall abundance, classification of the transposon or a specific site of genomic origin. The endo-siRNA biogenesis pathway in germline operates according to the same principles as the existing model for the soma, and its impairment does not significantly affect piRNAs. Expanding the analysis, we confirmed the occurrence of somatic piRNA-like RNAs (pilRNAs) that show a ping-pong signature. We detected expression of the Piwi-family protein mRNAs only barely above background, indicating that the somatic pilRNAs may arise from a small sub-population of somatic cells that express a functional piRNA pathway.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.