ArticlePloS one2014
Efficient and rapid induction of human iPSCs/ESCs into nephrogenic intermediate mesoderm using small molecule-based differentiation methods.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 119 citations in OpenAlex.
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- Nephron progenitors rhythmically alternate between renewal and differentiation phases that synchronize with kidney branching morphogenesis.bioRxiv : the preprint server for biology · 2024Article
- Generation of glucocorticoid-producing cells derived from human pluripotent stem cells.Cell reports methods · 2023Article
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- Opportunities and Challenges of Human IPSC Technology in Kidney Disease Research.Biomedicines · 2022Review
- Neuroblastoma SH-SY5Y Cell Differentiation to Mature Neuron by AM580 Treatment.Neurochemical research · 2022Article
- Current advances of stem cell-based therapy for kidney diseases.World journal of stem cells · 2021Review
- Testicular somatic cell-like cells derived from embryonic stem cells induce differentiation of epiblasts into germ cells.Communications biology · 2021Article
- Generation and characterization of iPSC-derived renal proximal tubule-like cells with extended stability.Scientific reports · 2021Article
- iPSC technology-based regenerative medicine for kidney diseases.Clinical and experimental nephrology · 2021Review
- Mapping Human Pluripotent Stem Cell-derived Erythroid Differentiation by Single-cell Transcriptome Analysis.Genomics, proteomics & bioinformatics · 2021Article
- Phenotypic technologies in stem cell biology.Cell chemical biology · 2021Review
- Human reconstructed kidney models.In vitro cellular & developmental biology. Animal · 2021Review
- High-Resolution Dissection of Chemical Reprogramming from Mouse Embryonic Fibroblasts into Fibrocartilaginous Cells.Stem cell reports · 2020Article
- Article
- Differentiating Induced Pluripotent Stem Cells into Renal Cells: A New Approach to Treat Kidney Diseases.Stem cells international · 2020Review
- Simple and Robust Differentiation of Human Pluripotent Stem Cells toward Chondrocytes by Two Small-Molecule Compounds.Stem cell reports · 2019Article
- Transient c-Src Suppression During Endodermal Commitment of Human Induced Pluripotent Stem Cells Results in Abnormal Profibrotic Cholangiocyte-Like Cells.Stem cells (Dayton, Ohio) · 2019Article
- Elongation of Müllerian ducts and connection to urogenital sinus determine the borderline of uterine and vaginal development.Biochemistry and biophysics reports · 2019Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The first step in developing regenerative medicine approaches to treat renal diseases using pluripotent stem cells must be the generation of intermediate mesoderm (IM), an embryonic germ layer that gives rise to kidneys. In order to achieve this goal, establishing an efficient, stable and low-cost method for differentiating IM cells using small molecules is required. In this study, we identified two retinoids, AM580 and TTNPB, as potent IM inducers by high-throughput chemical screening, and established rapid (five days) and efficient (80% induction rate) IM differentiation from human iPSCs using only two small molecules: a Wnt pathway activator, CHIR99021, combined with either AM580 or TTNPB. The resulting human IM cells showed the ability to differentiate into multiple cell types that constitute adult kidneys, and to form renal tubule-like structures. These small molecule differentiation methods can bypass the mesendoderm step, directly inducing IM cells by activating Wnt, retinoic acid (RA), and bone morphogenetic protein (BMP) pathways. Such methods are powerful tools for studying kidney development and may potentially provide cell sources to generate renal lineage cells for regenerative therapy.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.