ArticlePloS one2014
Novel genes participating in the formation of prismatic and nacreous layers in the pearl oyster as revealed by their tissue distribution and RNA interference knockdown.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 52 citations in OpenAlex.
- The Gln-rich protein twip1 promotes high-density {110} twins formation in the shell of the limpetProceedings of the National Academy of Sciences of the United States of America · 2025Article
- CRISPR/Cas9 Knockout of Shell Matrix Protein 1 in the Slipper-Snail Crepidula atrasolea.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2025Article
- Distribution of Conchocelis of Red Algae Bangia sp. in the Nacreous Layer of Akoya Pearl Oyster Pinctada fucata Shell.Marine biotechnology (New York, N.Y.) · 2025Article
- Ex Vivo Propagation of Pinctada Birnavirus Using Mantle Tissue Fragment Culture: Application for Measuring Replication at Different Temperatures, TCIDPathogens (Basel, Switzerland) · 2025Article
- The adult shell matrix protein repertoire of the marine snail Crepidula is dominated by conserved genes that are also expressed in larvae.BMC ecology and evolution · 2024Article
- Review
- EF-Hand-Binding Secreted Protein Hdh-SMP5 Regulates Shell Biomineralization and Responses to Stress in Pacific Abalone,Current issues in molecular biology · 2023Article
- Structural colors of pearls.Scientific reports · 2021Article
- Genome-wide DNA Methylation Analysis of Mantle Edge and Mantle Central from Pearl Oyster Pinctada fucata martensii.Marine biotechnology (New York, N.Y.) · 2020Article
- PfSMAD1/5 Can Interact with PfSMAD4 to Inhibit PfMSX to Regulate Shell Biomineralization in Pinctada fucata martensii.Marine biotechnology (New York, N.Y.) · 2020Article
- Pearl Sac Gene Expression Profiles Associated With Pearl Attributes in the Silver-Lip Pearl Oyster,Frontiers in genetics · 2020Article
- A Potential Role of Bone Morphogenetic Protein 7 in Shell Formation and Growth in the Razor ClamFrontiers in physiology · 2020Article
- Expression of calcification-related ion transporters during blue mussel larval development.Ecology and evolution · 2019Article
- Electroextraction of Insoluble Proteins from the Organic Matrix of the Nacreous Layer of the Japanese Pearl Oyster,Methods and protocols · 2019Article
- Gene expression profiles at different stages for formation of pearl sac and pearl in the pearl oyster Pinctada fucata.BMC genomics · 2019Article
- From the raw bar to the bench: Bivalves as models for human health.Developmental and comparative immunology · 2019Review
- Comparison of Two Pearl Sacs Formed in the Same Recipient Oyster with Different Genetic Background Involved in Yellow Pigmentation in Pinctada fucata.Marine biotechnology (New York, N.Y.) · 2018Article
- Cultured Pearl Surface Quality Profiling by the Shell Matrix Protein Gene Expression in the Biomineralised Pearl Sac Tissue of Pinctada margaritifera.Marine biotechnology (New York, N.Y.) · 2018Article
- Novel Isoforms of N16 and N19 Families Implicated for the Nacreous Layer Formation in the Pearl Oyster Pinctada fucata.Marine biotechnology (New York, N.Y.) · 2018Article
- The receptor genes PfBMPR1B and PfBAMBI are involved in regulating shell biomineralization in the pearl oyster Pinctada fucata.Scientific reports · 2017Article
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Authors and funding
13 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In our previous publication, we identified novel gene candidates involved in shell formation by EST analyses of the nacreous and prismatic layer-forming tissues in the pearl oyster Pinctada fucata. In the present study, 14 of those genes, including two known genes, were selected and further examined for their involvement in shell formation using the RNA interference. Molecular characterization based on the deduced amino acid sequences showed that seven of the novel genes encode secretory proteins. The tissue distribution of the transcripts of the genes, as analyzed by RT-PCR and in situ hybridization, was mostly consistent with those obtained by the EST analysis reported previously. Shells in the pearl oysters injected with dsRNAs targeting genes 000027, 000058, 000081, 000096, 000113 (nacrein), 000118, 000133 and 000411 (MSI60), which showed expression specific to the nacreous layer forming tissues, showed abnormal surface appearance in this layer. Individuals injected with dsRNAs targeting genes 000027, 000113 and 000133 also exhibited abnormal prismatic layers. Individuals injected with dsRNAs targeting genes 000031, 000066, 000098, 000145, 000194 and 000200, which showed expression specific to prismatic layer forming tissues, displayed an abnormal surface appearance in both the nacreous and prismatic layers. Taken together, the results suggest that the genes involved in prismatic layer formation might also be involved in the formation of the nacreous layers.
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