ArticlePloS one2014
The genome sequence of the fungal pathogen Fusarium virguliforme that causes sudden death syndrome in soybean.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 55 citations in OpenAlex.
- Genomic characterization and analysis of Fusarium Solani isolate 1-Jan, a novel pathogenic strain infecting Penaeus vannamei.Current genetics · 2025Article
- Genomic Differences Between TwoJournal of fungi (Basel, Switzerland) · 2025Article
- Soybean genomics research community strategic plan: A vision for 2024-2028.The plant genome · 2024Article
- Identification and molecular detection of the pathogen ofFrontiers in microbiology · 2024Article
- Chromosomal scale assembly reveals localized structural variants in avian caecal coccidian parasite Eimeria tenella.Scientific reports · 2023Article
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- Comparative genomics and association analysis identifies virulence genes of Cercospora sojina in soybean.BMC genomics · 2020Article
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- Comparative genomic analyses of two segregating mutants reveal seven genes likely involved in resistance to Fusarium equiseti in soybean via whole genome re-sequencing.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- A protoplast generation and transformation method for soybean sudden death syndrome causal agentsFungal biology and biotechnology · 2019Article
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- Fluopyram Sensitivity and Functional Characterization of SdhB in theFrontiers in microbiology · 2018Article
- Analysis of the genome sequence of Phomopsis longicolla: a fungal pathogen causing Phomopsis seed decay in soybean.BMC genomics · 2017Article
- Article
- Identification of the non-ribosomal peptide synthetase responsible for biosynthesis of the potential anti-cancer drug sansalvamide in Fusarium solani.Current genetics · 2016Article
- Genomic characterization of plant cell wall degrading enzymes and in silico analysis of xylanases and polygalacturonases of Fusarium virguliforme.BMC microbiology · 2016Article
- Black perithecial pigmentation in Fusarium species is due to the accumulation of 5-deoxybostrycoidin-based melanin.Scientific reports · 2016Article
- The Fusarium solani species complex: ubiquitous pathogens of agricultural importance.Molecular plant pathology · 2016Review
- Identification of Highly Variable Supernumerary Chromosome Segments in an Asexual Pathogen.PloS one · 2016Article
- Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
unlabelledFusarium virguliforme causes sudden death syndrome (SDS) of soybean, a disease of serious concern throughout most of the soybean producing regions of the world. Despite the global importance, little is known about the pathogenesis mechanisms of F. virguliforme. Thus, we applied Next-Generation DNA Sequencing to reveal the draft F. virguliforme genome sequence and identified putative pathogenicity genes to facilitate discovering the mechanisms used by the pathogen to cause this disease. METHODOLOGY/PRINCIPAL
findingsWe have generated the draft genome sequence of F. virguliforme by conducting whole-genome shotgun sequencing on a 454 GS-FLX Titanium sequencer. Initially, single-end reads of a 400-bp shotgun library were assembled using the PCAP program. Paired end sequences from 3 and 20 Kb DNA fragments and approximately 100 Kb inserts of 1,400 BAC clones were used to generate the assembled genome. The assembled genome sequence was 51 Mb. The N50 scaffold number was 11 with an N50 Scaffold length of 1,263 Kb. The AUGUSTUS gene prediction program predicted 14,845 putative genes, which were annotated with Pfam and GO databases. Gene distributions were uniform in all but one of the major scaffolds. Phylogenic analyses revealed that F. virguliforme was closely related to the pea pathogen, Nectria haematococca. Of the 14,845 F. virguliforme genes, 11,043 were conserved among five Fusarium species: F. virguliforme, F. graminearum, F. verticillioides, F. oxysporum and N. haematococca; and 1,332 F. virguliforme-specific genes, which may include pathogenicity genes. Additionally, searches for candidate F. virguliforme pathogenicity genes using gene sequences of the pathogen-host interaction database identified 358 genes.
conclusionsThe F. virguliforme genome sequence and putative pathogenicity genes presented here will facilitate identification of pathogenicity mechanisms involved in SDS development. Together, these resources will expedite our efforts towards discovering pathogenicity mechanisms in F. virguliforme. This will ultimately lead to improvement of SDS resistance in soybean.
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