ArticlePloS one2014
Microarray analysis of the Ler regulon in enteropathogenic and enterohaemorrhagic Escherichia coli strains.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- Article
- GrlR, a negative regulator in enteropathogenicFrontiers in microbiology · 2023Article
- Selected commensals educate the intestinal vascular and immune system for immunocompetence.Microbiome · 2022Article
- Regulatory Evolution of theJournal of bacteriology · 2022Article
- Characterization of the Mode of Action of Aurodox, a Type III Secretion System Inhibitor from Streptomyces goldiniensis.Infection and immunity · 2019Article
- Plasma membrane profiling during enterohemorrhagicThe Journal of biological chemistry · 2018Article
- Article
- Sulfamethoxazole - Trimethoprim represses csgD but maintains virulence genes at 30°C in a clinical Escherichia coli O157:H7 isolate.PloS one · 2018Article
- Environment Controls LEE Regulation in EnteropathogenicFrontiers in microbiology · 2018Review
- Article
- Novel compounds targeting the enterohemorrhagic Escherichia coli type three secretion system reveal insights into mechanisms of secretion inhibition.Molecular microbiology · 2017Article
- Regulation ofEcoSal Plus · 2017Review
- PerC Manipulates Metabolism and Surface Antigens in EnteropathogenicFrontiers in cellular and infection microbiology · 2017Article
- Lambda Red-mediated Recombineering in the Attaching and Effacing Pathogen Escherichia albertii.Biological procedures online · 2016Article
- A Highly Conserved Bacterial D-Serine Uptake System Links Host Metabolism and Virulence.PLoS pathogens · 2016Article
- RNA-Seq analysis of isolate- and growth phase-specific differences in the global transcriptomes of enteropathogenic Escherichia coli prototype isolates.Frontiers in microbiology · 2015Article
- The gut bacterium Bacteroides thetaiotaomicron influences the virulence potential of the enterohemorrhagic Escherichia coli O103:H25.PloS one · 2015Article
- Dynamics of expression and maturation of the type III secretion system of enteropathogenic Escherichia coli.Journal of bacteriology · 2014Article
- High variation acrossGut microbesArticle
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
The type III protein secretion system is an important pathogenicity factor of enteropathogenic and enterohaemorrhagic Escherichia coli pathotypes. The genes encoding this apparatus are located on a pathogenicity island (the locus of enterocyte effacement) and are transcriptionally activated by the master regulator Ler. In each pathotype Ler is also known to regulate genes located elsewhere on the chromosome, but the full extent of the Ler regulon is unclear, especially for enteropathogenic E. coli. The Ler regulon was defined for two strains of E. coli: E2348/69 (enteropathogenic) and EDL933 (enterohaemorrhagic) in mid and late log phases of growth by DNA microarray analysis of the transcriptomes of wild-type and ler mutant versions of each strain. In both strains the Ler regulon is focused on the locus of enterocyte effacement - all major transcriptional units of which are activated by Ler, with the sole exception of the LEE1 operon during mid-log phase growth in E2348/69. However, the Ler regulon does extend more widely and also includes unlinked pathogenicity genes: in E2348/69 more than 50 genes outside of this locus were regulated, including a number of known or potential pathogenicity determinants; in EDL933 only 4 extra-LEE genes, again including known pathogenicity factors, were activated. In E2348/69, where the Ler regulon is clearly growth phase dependent, a number of genes including the plasmid-encoded regulator operon perABC, were found to be negatively regulated by Ler. Negative regulation by Ler of PerC, itself a positive regulator of the ler promoter, suggests a negative feedback loop involving these proteins.
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