ArticleIranian journal of parasitology2013
Immunoproteomic Analysis of the Excretory-Secretory Proteins from Spirometra mansoni Sparganum.
Article in Iranian journal of parasitology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- Proteomic and Immunological Identification of Diagnostic Antigens from Spirometra erinaceieuropaei Plerocercoid.The Korean journal of parasitology · 2021Article
- Proteomic Insights into the Biology of the Most Important Foodborne Parasites in Europe.Foods (Basel, Switzerland) · 2020Review
- Protein phosphorylation networks in spargana of Spirometra erinaceieuropaei revealed by phosphoproteomic analysis.Parasites & vectors · 2020Article
- Efficacy comparison between long-term high-dose praziquantel and surgical therapy for cerebral sparganosis: A multicenter retrospective cohort study.PLoS neglected tropical diseases · 2018Article
- Identification of 24h Ixodes scapularis immunogenic tick saliva proteins.Ticks and tick-borne diseases · 2015Article
- Serodiagnosis of sparganosis by ELISA using recombinant cysteine protease of Spirometra erinaceieuropaei spargana.Parasitology research · 2015Article
- Characterization of Spirometra erinaceieuropaei Plerocercoid Cysteine Protease and Potential Application for Serodiagnosis of Sparganosis.PLoS neglected tropical diseases · 2015Article
- Phylogenetic Location of the Spirometra Sparganum Isolates from China, Based on Sequences of 28S rDNA D1.Iranian journal of parasitology · 2014Article
- Molecular characterization of a Spirometra mansoni antigenic polypeptide gene encoding a 28.7 kDa protein.Parasitology research · 2014Article
- A 24-48 h fed Amblyomma americanum tick saliva immuno-proteome.BMC genomics · 2014Article
- Analysis of structures, functions, and epitopes of cysteine protease from Spirometra erinaceieuropaei Spargana.BioMed research international · 2013Article
- The Use of Recombinant 31 kDa Antigens of Trichinella spiralis for Serodiagnosis of Experimental Trichinellosis.Iranian journal of parasitologyArticle
- Preliminary Analysis of Taenia multiceps Metacestode Antigens by Two-Dimensional Electrophoresis.Iranian journal of parasitologyArticle
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSparganosis is caused by the invasion of Spirometra sparganum into various tissues/organs. Subcutaneous sparganosis can be diagnosed by biopsy, while visceral/cerebral sparganosis is not easy to be diagnosed. The diagnosis depends largely on the detection of specific anti-sparganum antibodies. The specificity of the ELISA could be increased by using S. mansoni sparganum excretory-secretory (ES) antigens, but it also had the cross-reactions with sera of patients with cysticercosis or paragonimiasis. The aim of this study was to identify early specific diagnostic antigens in S. mansoni sparganum ES proteins.
methodsThe sparganum ES proteins were analyzed by two-dimensional electrophoresis (2-DE) and Western blot probed with early sera from infected mice at 14 days post-infection. The immunoreactive protein spots were characterized by MALDI-TOF/ TOF-MS.
resultsA total of approximately 149 proteins spots were detected with isoelectric point (pI) varying from 3 to 7.5 and molecular weight from 20 to 115 kDa and seven protein spots with molecular weight of 23-31 kDa were recognized by the infection sera. Three of seven spots were successfully identified and characterized as the same S. mansoni protein (cysteine protease), and the proteins of other 4 spots were not included in the databases.
conclusionThe cysteine protease from S. mansoni ES proteins recognized by early infection sera might be the early diagnostic antigens for sparganosis.
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24454434PMC3887242W2185438412What OpenQuestion holds
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