ArticleFrontiers in plant science2014
Using transcription of six Puccinia triticina races to identify the effective secretome during infection of wheat.
Article in Frontiers in plant science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 68 citations in OpenAlex.
- Mechanism of Virulence in Fungal Pathogens with Diverse Modes of Living.Current microbiology · 2026Review
- The proteome study of germinated Puccinia triticina urediniospores reveals a novel effector protein required for virulence.Scientific reports · 2026Article
- Wheat Leaf Rust Effector Pt48115 Localized in the Chloroplasts and Suppressed Wheat Immunity.Journal of fungi (Basel, Switzerland) · 2025Article
- The Underexplored Mechanisms of Wheat Resistance to Leaf Rust.Plants (Basel, Switzerland) · 2023Review
- Molecular Mechanisms of the Co-Evolution of Wheat and Rust Pathogens.Plants (Basel, Switzerland) · 2023Review
- Candidate effectors for leaf rust resistance geneFrontiers in microbiology · 2023Article
- Article
- Infection Strategies and Pathogenicity of Biotrophic Plant Fungal Pathogens.Frontiers in microbiology · 2022Review
- Wheat leaf rust fungus effector Pt13024 is avirulent to TcLr30.Frontiers in plant science · 2022Article
- Comparative Transcriptomics and RNA-Seq-Based Bulked Segregant Analysis Reveals Genomic Basis UnderlyingFrontiers in microbiology · 2021Article
- Article
- Smut fungi as a stratagem to characterize rust effectors: opportunities and challenges.World journal of microbiology & biotechnology · 2020Review
- Puccinia triticina pathotypes THTT and THTS display complex transcript profiles on wheat cultivar Thatcher.BMC genetics · 2020Article
- Long-Read-BasedFrontiers in genetics · 2020Article
- Predicating the Effector Proteins Secreted byFrontiers in microbiology · 2020Article
- Genome-Wide Identification of Effector Candidates With Conserved Motifs From the Wheat Leaf Rust FungusFrontiers in microbiology · 2020Article
- Transcriptome-wide association study identifies putative elicitors/suppressor of Puccinia graminis f. sp. tritici that modulate barley rpg4-mediated stem rust resistance.BMC genomics · 2019Article
- Review
- A review of wheat diseases-a field perspective.Molecular plant pathology · 2018Review
- Article
Corrections and comments
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Authors and funding
10 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Wheat leaf rust, caused by the basidiomycete Puccinia triticina, can cause yield losses of up to 20% in wheat producing regions. During infection, the fungus forms haustoria that secrete proteins into the plant cell and effect changes in plant transcription, metabolism, and defense. It is hypothesized that new races emerge as a result of overcoming plant resistance via changes in the secreted effector proteins. To understand gene expression during infection and find genetic differences associated with races, RNA from wheat leaves infected with six different rust races, at 6 days post inoculation, was sequenced using Illumina. As P. triticina is an obligate biotroph, RNA from both the host and fungi were present and separated by alignment to the P. triticina genome and a wheat EST reference. A total of 222,571 rust contigs were assembled from 165 million reads. An examination of the resulting contigs revealed 532 predicted secreted proteins among the transcripts. Of these, 456 were found in all races. Fifteen genes were found with amino acid changes, corresponding to putative avirulence effectors potentially recognized by 11 different leaf rust resistance (Lr) genes. Twelve of the potential avirulence effectors have no homology to known genes. One gene had significant similarity to cerato-platanin, a known fungal elicitor, and another showed similarity to fungal tyrosinase, an enzyme involved in melanin synthesis. Temporal expression profiles were developed for these genes by qRT-PCR and show that the genes expression patterns were consistent between races from infection initiation to just prior to spore eruption.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.