ArticlePLoS genetics2014
Genomic confirmation of hybridisation and recent inbreeding in a vector-isolated Leishmania population.
Article in PLoS genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.
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Who cites it
87 citing papers in PubMed, 177 citations in OpenAlex.
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- TIM, a novel molecular toolbox for the detection and identification ofMicrobiology spectrum · 2026Article
- Whole genome sequencing reveals genomic diversity and evidence of hybridization in MoroccanFrontiers in cellular and infection microbiology · 2026Article
- Trypanosomatid parasites, translational research in a One Health, One World environment.Frontiers in chemistry · 2026Review
- Epidemiological dynamics ofMicrobial genomics · 2025Article
- Partners in time: Cospeciation and host-switching shape the evolution of Leishmania parasites.PLoS neglected tropical diseases · 2025Article
- Unraveling the Genome Diversity ofLife (Basel, Switzerland) · 2025Review
- At the genetic crossroads of Leishmania: Emerging hybrids reshaping disease patterns.PLoS pathogens · 2025Article
- Combination of Cpb-Hsp70 typing methods reveals genetic divergence between Leishmania infantum strains causing human tegumentary leishmaniasis in northern Italy and central Spain: a retrospective study.Infectious diseases of poverty · 2025Article
- Susceptibility of diverse sand fly species to Toscana virus.PLoS neglected tropical diseases · 2025Article
- Cross-subgenus hybridization betweenbioRxiv : the preprint server for biology · 2025Article
- BALB/c mice infection with hybridFrontiers in cellular and infection microbiology · 2025Article
- Understanding the genetic complexity of Leishmania infantum in the Americas: a focus on 3'NT/NU gene deletion.Memorias do Instituto Oswaldo Cruz · 2025Review
- A novel strain of Leishmania braziliensis harbors not a toti- but a bunyavirus.PLoS neglected tropical diseases · 2024Article
- Evolutionary genomics of Leishmania braziliensis across the neotropical realm.Communications biology · 2024Article
- The development of L. major, L. donovani and L. martiniquensis, Leishmania currently emerging in Europe, in the sand fly species Phlebotomus perniciosus and P. tobbi.PLoS neglected tropical diseases · 2024Article
- Epidemiological and clinical trends of visceral leishmaniasis in Portugal: retrospective analysis of cases diagnosed in public hospitals between 2010 and 2020.Infectious diseases of poverty · 2024Article
- Geographical and Molecular Analysis of Haplotype Variations in Leishmania major Among Infected Iranian Phlebotomus papatasi.Acta parasitologica · 2024Article
- Comparative genomics of Leishmania donovani progeny from genetic crosses in two sand fly species and impact on the diversity of diagnostic and vaccine candidates.PLoS neglected tropical diseases · 2024Article
27 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
Abstract
Although asexual reproduction via clonal propagation has been proposed as the principal reproductive mechanism across parasitic protozoa of the Leishmania genus, sexual recombination has long been suspected, based on hybrid marker profiles detected in field isolates from different geographical locations. The recent experimental demonstration of a sexual cycle in Leishmania within sand flies has confirmed the occurrence of hybridisation, but knowledge of the parasite life cycle in the wild still remains limited. Here, we use whole genome sequencing to investigate the frequency of sexual reproduction in Leishmania, by sequencing the genomes of 11 Leishmania infantum isolates from sand flies and 1 patient isolate in a focus of cutaneous leishmaniasis in the Çukurova province of southeast Turkey. This is the first genome-wide examination of a vector-isolated population of Leishmania parasites. A genome-wide pattern of patchy heterozygosity and SNP density was observed both within individual strains and across the whole group. Comparisons with other Leishmania donovani complex genome sequences suggest that these isolates are derived from a single cross of two diverse strains with subsequent recombination within the population. This interpretation is supported by a statistical model of the genomic variability for each strain compared to the L. infantum reference genome strain as well as genome-wide scans for recombination within the population. Further analysis of these heterozygous blocks indicates that the two parents were phylogenetically distinct. Patterns of linkage disequilibrium indicate that this population reproduced primarily clonally following the original hybridisation event, but that some recombination also occurred. This observation allowed us to estimate the relative rates of sexual and asexual reproduction within this population, to our knowledge the first quantitative estimate of these events during the Leishmania life cycle.
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