ArticlePLoS genetics2014
A comprehensive tRNA deletion library unravels the genetic architecture of the tRNA pool.
Article in PLoS genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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Who cites it
67 citing papers in PubMed, 92 citations in OpenAlex.
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- Essentiality and dynamic expression of the human tRNA pool during viral infection.Molecular systems biology · 2026Article
- Hidden Diversity in Yeast tRNAs: Comparative Genomics and Modification Mapping in a Eukaryotic Subphylum.bioRxiv : the preprint server for biology · 2026Article
- Allelic Variation at tRNA Genes in Three Nematode Species Indicates Mutation Load Despite Strong Purifying Selection.Genome biology and evolution · 2026Article
- How Does Transcription-Associated Mutagenesis Shape tRNA Microevolution?Genome biology and evolution · 2026Article
- Coupling tRNAGly gene redundancy with staphylococcal cell wall integrity, antibiotic susceptibility, and virulence potential.Nucleic acids research · 2025Article
- A machine learning approach uncovers principles and determinants of eukaryotic ribosome pausing.Science advances · 2024Article
- Systematic analysis of tRNA transcription unit deletions in E. coli reveals insights into tRNA gene essentiality and cellular adaptation.Scientific reports · 2024Article
- Disruption of tRNA biogenesis enhances proteostatic resilience, improves later-life health, and promotes longevity.PLoS biology · 2024Article
- The choreography of chromatin in RNA polymerase III regulation.Biochemical Society transactions · 2024Review
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- Tuning tRNAs for improved translation.Frontiers in genetics · 2024Review
- Locus-specific proteome decoding reveals Fpt1 as a chromatin-associated negative regulator of RNA polymerase III assembly.Molecular cell · 2023Article
- Establishing chromosomal design-build-test-learn through a synthetic chromosome and its combinatorial reconfiguration.Cell genomics · 2023Article
- Copy number variation in tRNA isodecoder genes impairs mammalian development and balanced translation.Nature communications · 2023Article
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- Anticodon sequence determines the impact of mistranslating tRNARNA biology · 2023Article
- Disproportionate investment in Spiralin B production limits in-host growth and favors the vertical transmission ofProceedings of the National Academy of Sciences of the United States of America · 2022Article
7 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Deciphering the architecture of the tRNA pool is a prime challenge in translation research, as tRNAs govern the efficiency and accuracy of the process. Towards this challenge, we created a systematic tRNA deletion library in Saccharomyces cerevisiae, aimed at dissecting the specific contribution of each tRNA gene to the tRNA pool and to the cell's fitness. By harnessing this resource, we observed that the majority of tRNA deletions show no appreciable phenotype in rich medium, yet under more challenging conditions, additional phenotypes were observed. Robustness to tRNA gene deletion was often facilitated through extensive backup compensation within and between tRNA families. Interestingly, we found that within tRNA families, genes carrying identical anti-codons can contribute differently to the cellular fitness, suggesting the importance of the genomic surrounding to tRNA expression. Characterization of the transcriptome response to deletions of tRNA genes exposed two disparate patterns: in single-copy families, deletions elicited a stress response; in deletions of genes from multi-copy families, expression of the translation machinery increased. Our results uncover the complex architecture of the tRNA pool and pave the way towards complete understanding of their role in cell physiology.
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