ArticlePLoS biology2014
Electron tomography and simulation of baculovirus actin comet tails support a tethered filament model of pathogen propulsion.
Article in PLoS biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 54 citations in OpenAlex.
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- Mechanistic insights into actin force generation during vesicle formation from cryo-electron tomography.Developmental cell · 2022Article
- Ebola Virus Nucleocapsid-Like Structures Utilize Arp2/3 Signaling for Intracellular Long-Distance Transport.Cells · 2020Article
- Three-dimensional organization of the cytoskeleton: A cryo-electron tomography perspective.Protein science : a publication of the Protein Society · 2020Review
- High Rac1 activity is functionally translated into cytosolic structures with unique nanoscale cytoskeletal architecture.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
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- Exploitation of Cytoskeletal Networks during Early Viral Infection.Trends in microbiology · 2019Review
- Baculovirus Actin-Based Motility Drives Nuclear Envelope Disruption and Nuclear Egress.Current biology : CB · 2018Article
- Autographa californica Multiple Nucleopolyhedrovirus Ac34 Protein Retains Cellular Actin-Related Protein 2/3 Complex in the Nucleus by Subversion of CRM1-Dependent Nuclear Export.PLoS pathogens · 2016Article
- Combined optical micromanipulation and interferometric topography (COMMIT).Biomedical optics express · 2016Article
- Quantitative Analysis of Filament Branch Orientation in Listeria Actin Comet Tails.Biophysical journal · 2016Article
- Integration of linear and dendritic actin nucleation in Nck-induced actin comets.Molecular biology of the cell · 2016Article
- Trichoplusia ni Kinesin-1 Associates with Autographa californica Multiple Nucleopolyhedrovirus Nucleocapsid Proteins and Is Required for Production of Budded Virus.Journal of virology · 2016Article
- Isoform diversity in the Arp2/3 complex determines actin filament dynamics.Nature cell biology · 2016Article
- Viral Infection at High Magnification: 3D Electron Microscopy Methods to Analyze the Architecture of Infected Cells.Viruses · 2015Review
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13 authors at 7 institutions in 4 countries.
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Abstract
Several pathogens induce propulsive actin comet tails in cells they invade to disseminate their infection. They achieve this by recruiting factors for actin nucleation, the Arp2/3 complex, and polymerization regulators from the host cytoplasm. Owing to limited information on the structural organization of actin comets and in particular the spatial arrangement of filaments engaged in propulsion, the underlying mechanism of pathogen movement is currently speculative and controversial. Using electron tomography we have resolved the three-dimensional architecture of actin comet tails propelling baculovirus, the smallest pathogen yet known to hijack the actin motile machinery. Comet tail geometry was also mimicked in mixtures of virus capsids with purified actin and a minimal inventory of actin regulators. We demonstrate that propulsion is based on the assembly of a fishbone-like array of actin filaments organized in subsets linked by branch junctions, with an average of four filaments pushing the virus at any one time. Using an energy-minimizing function we have simulated the structure of actin comet tails as well as the tracks adopted by baculovirus in infected cells in vivo. The results from the simulations rule out gel squeezing models of propulsion and support those in which actin filaments are continuously tethered during branch nucleation and polymerization. Since Listeria monocytogenes, Shigella flexneri, and Vaccinia virus among other pathogens use the same common toolbox of components as baculovirus to move, we suggest they share the same principles of actin organization and mode of propulsion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.