ArticleActa ortopedica brasileira2013
In vitro effects of caffeine in growth cartilage of rats.
Article in Acta ortopedica brasileira, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 5 citations in OpenAlex.
- Effects of Caffeine on Dental Mesenchymal Stem Cells: Implications for Regenerative Applications.Journal of functional biomaterials · 2025Article
- Effects of Caffeine on Intervertebral Disc Cell Viability in a Whole Organ Culture Model.Global spine journal · 2022Article
- Retinoic Acid Signaling Plays a Crucial Role in Excessive Caffeine Intake-Disturbed Apoptosis and Differentiation of Myogenic Progenitors.Frontiers in cell and developmental biology · 2021Article
- Caffeine, a Risk Factor for Osteoarthritis and Longitudinal Bone Growth Inhibition.Journal of clinical medicine · 2020Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo evaluate the in vitro effetcs of caffeine on proliferation, apoptosis a nd gene transcripts expression of chondrogenic differentiation in growth cartilage.
methodsTHE CARTILAGINOUS EPIPHYSES OF FEMURS OF NEWBORN RATS, WHICH WERE DIVIDED INTO TWO SUBGROUPS: treated with caffeine and control group, both observed over the time periods of 0, 7, 14 and 21 days. The cartilaginous epiphyses of femurs of each subgroup and each time span were subjected to histomorphometric, immunohistochemical analysis, Tunel technique and RT-PCR in real time.
resultsThe decrease in proliferative activity and the increase of apoptotic chondroblasts at 21 days were found regardless of the subgroup. However, the decrease in cell proliferation caused by caffeine was lower than in the control group and significantly increased the expression of gene transcripts for chondrogenic differentiation, represented by Sox-9 and Runx-2. However, the in vitro culture with caffeine revealed antagonistic effects: despite the positive effect on chondroblasts proliferation and differentiation, caffeine increased apoptosis, characterized by increased expression of caspase 3 and of the number of cells undergoing apoptosis (p<0.05).
conclusionCaffeine presents antagonistic effects in vitro on growth cartilage, increasing the proliferation, differentiation and cell apoptosis. Experimental Study .
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