ArticleMicrobial ecology2014
Listeria monocytogenes can form biofilms in tap water and enter into the viable but non-cultivable state.
Article in Microbial ecology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 56 citations in OpenAlex.
- Phenotypic Profiling of Biofilm Formation and Antibiotic Susceptibility in Poultry-DerivedAntibiotics (Basel, Switzerland) · 2026Article
- Nano-coating with silicon dioxide to reduce the occurrence of bacterial contamination in a pig abattoir drinking system.Folia microbiologica · 2025Article
- Aquatic environment drives the emergence of cell wall-deficient dormant forms in Listeria.Nature communications · 2024Article
- Review
- The Viable But Non-Culturable State ofFrontiers in cellular and infection microbiology · 2022Review
- Identification of the key functional genes in salt-stress tolerance of Cyanobacterium3 Biotech · 2021Article
- Challenges of antibiotic resistance biofilms and potential combating strategies: a review.3 Biotech · 2021Review
- Detection and Potential Virulence of Viable but Non-Culturable (VBNC)Microorganisms · 2021Review
- Heat Resistance ofFrontiers in microbiology · 2020Article
- Evaluation of Enzymatic Cleaning on Food Processing Installations and Food Products Bacterial Microflora.Frontiers in microbiology · 2020Article
- Propidium iodide staining underestimates viability of adherent bacterial cells.Scientific reports · 2019Article
- Preharvest Farming Practices Impacting Fresh Produce Safety.Microbiology spectrum · 2018Review
- Article
- Biofilms in the Food Industry: Health Aspects and Control Methods.Frontiers in microbiology · 2018Review
- Current Perspectives on Viable but Non-culturable State in Foodborne Pathogens.Frontiers in microbiology · 2017Review
- The importance of the viable but non-culturable state in human bacterial pathogens.Frontiers in microbiology · 2014Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Listeria monocytogenes is a foodborne pathogen that can be transmitted through contaminated raw food or by ready-to-eat products that have been in contact with contaminated surfaces. Tap water (TW) is used to wash produce, as a processed food constituent and to wash processing surfaces and floors. The main aim of this work was to investigate the formation and survival of L. monocytogenes biofilms on stainless steel (SS) coupons in TW at 4, 22, 30 and 37 °C. For that, coupons with biofilm were visualised in situ while other coupons were scraped to quantify total cells by SYTO 9, cultivable numbers by plating onto brain heart infusion agar and viable numbers by the direct viable count method. Results showed that L. monocytogenes can form biofilms on SS surfaces in TW at any temperature, including at 4 °C. The number of total cells was similar for all the conditions tested while cultivable numbers varied between the level of detection (<8.3 CFU cm(-2)) and 3.5 × 10(5) CFU cm(-2), meaning between 7.0 × 10(4) and 1.1 × 10(7) cells cm(-2) have entered the viable but non-cultivable (VBNC) state. This work clearly demonstrates that L. monocytogenes can form biofilms in TW and that sessile cells can remain viable and cultivable in some conditions for at least the 48 h investigated. On the other hand, VBNC adaptation suggests that the pathogen can remain undetectable using traditional culture recovery techniques, which may give a false indication of processing surface hygiene status, leading to potential cross-contamination of food products.
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