ArticleNucleic acids research2014
Adjacent single-stranded regions mediate processing of tRNA precursors by RNase E direct entry.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 37 citations in OpenAlex.
- Bacterial/archaeal protein-only RNase P: complementation inRNA (New York, N.Y.) · 2025Article
- An sRNA overexpression library reveals AbnZ as a negative regulator of an essential translocation module in Caulobacter crescentus.Nucleic acids research · 2025Article
- Cooperation of regulatory RNA and the RNA degradosome in transcript surveillance.Nucleic acids research · 2024Article
- A conserved protein inhibitor brings under check the activity of RNase E in cyanobacteria.Nucleic acids research · 2024Article
- The role of the 5' sensing function of ribonuclease E in cyanobacteria.RNA biology · 2024Article
- The Fate and Functionality of Alien tRNA Fragments in Culturing Medium and Cells ofInternational journal of molecular sciences · 2023Article
- Structure of a bacterial ribonucleoprotein complex central to the control of cell envelope biogenesis.The EMBO journal · 2023Article
- The recognition of structured elements by a conserved groove distant from domains associated with catalysis is an essential determinant of RNase E.Nucleic acids research · 2023Article
- Regulation of mRNA decay inCritical reviews in biochemistry and molecular biology · 2022Article
- Inactivation of RNase P in Escherichia coli significantly changes post-transcriptional RNA metabolism.Molecular microbiology · 2022Article
- Transcriptome-wide in vivo mapping of cleavage sites for the compact cyanobacterial ribonuclease E reveals insights into its function and substrate recognition.Nucleic acids research · 2021Article
- Impact of pseudouridylation, substrate fold, and degradosome organization on the endonuclease activity of RNase E.RNA (New York, N.Y.) · 2021Article
- Adaptor protein RapZ activates endoribonuclease RNase E by protein-protein interaction to cleave a small regulatory RNA.RNA (New York, N.Y.) · 2020Article
- Cross-subunit catalysis and a new phenomenon of recessive resurrection in Escherichia coli RNase E.Nucleic acids research · 2020Article
- Bacterial RNA Degradosomes: Molecular Machines under Tight Control.Trends in biochemical sciences · 2020Review
- Substrate Recognition and Autoinhibition in the Central Ribonuclease RNase E.Molecular cell · 2018Article
- Enzymes Involved in Posttranscriptional RNA Metabolism in Gram-Negative Bacteria.Microbiology spectrum · 2018Review
- RNase E and the High-Fidelity Orchestration of RNA Metabolism.Microbiology spectrum · 2018Review
- TheJournal of bacteriology · 2017Article
- Downstream element determines RNase Y cleavage of the saePQRS operon in Staphylococcus aureus.Nucleic acids research · 2017Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The RNase E family is renowned for being central to the processing and decay of all types of RNA in many species of bacteria, as well as providing the first examples of endonucleases that can recognize 5'-monophosphorylated ends thereby increasing the efficiency of cleavage. However, there is increasing evidence that some transcripts can be cleaved efficiently by Escherichia coli RNase E via direct entry, i.e. in the absence of the recognition of a 5'-monophosphorylated end. Here, we provide biochemical evidence that direct entry is central to the processing of transfer RNA (tRNA) in E. coli, one of the core functions of RNase E, and show that it is mediated by specific unpaired regions that are adjacent, but not contiguous to segments cleaved by RNase E. In addition, we find that direct entry at a site on the 5' side of a tRNA precursor triggers a series of 5'-monophosphate-dependent cleavages. Consistent with a major role for direct entry in tRNA processing, we provide additional evidence that a 5'-monophosphate is not required to activate the catalysis step in cleavage. Other examples of tRNA precursors processed via direct entry are also provided. Thus, it appears increasingly that direct entry by RNase E has a major role in bacterial RNA metabolism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.