ArticleParasites & vectors2014
Tick capillary feeding for the study of proteins involved in tick-pathogen interactions as potential antigens for the control of tick infestation and pathogen infection.
Article in Parasites & vectors, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 49 citations in OpenAlex.
- Exploring the Combined Effect of Bm86 and Subolesin Polypeptide Vaccines in Cattle Naturally Infested withVeterinary sciences · 2026Article
- Functional annotation and analysis of the hard tick Dermacentor nuttalli midgut genes.Parasitology research · 2025Article
- Review
- Article
- In vitro feeding of Hyalomma excavatum and Hyalomma marginatum tick species.Parasitology research · 2023Article
- Advances inPathogens (Basel, Switzerland) · 2023Review
- Changes in theFrontiers in microbiology · 2022Article
- Challenges in Veterinary Vaccine Development.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Experimental Infection of Ticks: An Essential Tool for the Analysis ofPathogens (Basel, Switzerland) · 2021Review
- Artificial Feeding of All Consecutive Life Stages ofVaccines · 2021Article
- Tick Importin-α Is Implicated in the Interactome and Regulome of the Cofactor Subolesin.Pathogens (Basel, Switzerland) · 2021Article
- Cocktail Anti-Tick Vaccines: The Unforeseen Constraints and Approaches toward Enhanced Efficacies.Vaccines · 2020Review
- Understanding Tick Biology and Its Implications in Anti-tick and Transmission Blocking Vaccines Against Tick-Borne Pathogens.Frontiers in veterinary science · 2020Article
- Tick Cell Lines in Research on Tick Control.Frontiers in physiology · 2020Review
- A combination of antibodies against Bm86 and Subolesin inhibits engorgement of Rhipicephalus australis (formerly Rhipicephalus microplus) larvae in vitro.Parasites & vectors · 2019Article
- Differential expression analysis for subolesin in Rhipicephalus microplus infected with Anaplasma marginale.Experimental & applied acarology · 2018Article
- The Tick Cell Biobank: A global resource for in vitro research on ticks, other arthropods and the pathogens they transmit.Ticks and tick-borne diseases · 2018Review
- Identification of Lyme borreliae proteins promoting vertebrate host blood-specific spirochete survival in Ixodes scapularis nymphs using artificial feeding chambers.Ticks and tick-borne diseases · 2018Article
- The Use of Ex Vivo Organ Cultures in Tick-Borne Virus Research.ACS infectious diseases · 2018Review
- Functional Evolution of Subolesin/Akirin.Frontiers in physiology · 2018Review
Corrections and comments
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Authors and funding
11 authors at 7 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTicks represent a significant health risk to animals and humans due to the variety of pathogens they can transmit during feeding. The traditional use of chemicals to control ticks has serious drawbacks, including the selection of acaricide-resistant ticks and environmental contamination with chemical residues. Vaccination with the tick midgut antigen BM86 was shown to be a good alternative for cattle tick control. However, results vary considerably between tick species and geographic location. Therefore, new antigens are required for the development of vaccines controlling both tick infestations and pathogen infection/transmission. Tick proteins involved in tick-pathogen interactions may provide good candidate protective antigens for these vaccines, but appropriate screening procedures are needed to select the best candidates.
methodsIn this study, we selected proteins involved in tick-Anaplasma (Subolesin and SILK) and tick-Babesia (TROSPA) interactions and used in vitro capillary feeding to characterize their potential as antigens for the control of cattle tick infestations and infection with Anaplasma marginale and Babesia bigemina. Purified rabbit polyclonal antibodies were generated against recombinant SUB, SILK and TROSPA and added to uninfected or infected bovine blood to capillary-feed female Rhipicephalus (Boophilus) microplus ticks. Tick weight, oviposition and pathogen DNA levels were determined in treated and control ticks.
resultsThe specificity of purified rabbit polyclonal antibodies against tick recombinant proteins was confirmed by Western blot and against native proteins in tick cell lines and tick tissues using immunofluorescence. Capillary-fed ticks ingested antibodies added to the blood meal and the effect of these antibodies on tick weight and oviposition was shown. However, no effect was observed on pathogen DNA levels.
conclusionsThese results highlighted the advantages and some of the disadvantages of in vitro tick capillary feeding for the characterization of candidate tick protective antigens. While an effect on tick weight and oviposition was observed, the effect on pathogen levels was not evident probably due to high tick-to-tick variations among other factors. Nevertheless, these results together with previous results of RNA interference functional studies suggest that these proteins are good candidate vaccine antigens for the control of R. microplus infestations and infection with A. marginale and B. bigemina.
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