ArticleBMC pediatrics2014
Increased IL-4 mRNA expression and poly-aromatic hydrocarbon concentrations from children with asthma.
Article in BMC pediatrics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 17 citations in OpenAlex.
- The Association between Vitamin D, Interleukin-4, and Interleukin-10 Levels and CD23+ Expression with Bronchial Asthma in Stunted Children.Biomedicines · 2023Article
- Multidimensional Analysis of Lung Lymph Nodes in a Mouse Model of Allergic Lung Inflammation followingEnvironmental health perspectives · 2023Article
- The Mechanisms of Effector Th Cell Responses Contribute to Treg Cell Function: New Insights into Pathogenesis and Therapy of Asthma.Frontiers in immunology · 2022Review
- Barrier Impairment and Type 2 Inflammation in Allergic Diseases: The Pediatric Perspective.Children (Basel, Switzerland) · 2021Review
- The altered immunological status of children conceived by assisted reproductive technology.Reproductive biology and endocrinology : RB&E · 2021Article
- Network and co-expression analysis of airway smooth muscle cell transcriptome delineates potential gene signatures in asthma.Scientific reports · 2021Article
- Interactions between polycyclic aromatic hydrocarbons and epoxide hydrolase 1 play roles in asthma.Environmental geochemistry and health · 2019Review
- Antiasthmatic Effects of Sanglong Pingchuan Decoction through Inducing a Balanced Th1/Th2 Immune Response.Evidence-based complementary and alternative medicine : eCAM · 2018Article
- Proximity to a Major Road and Plasma Cytokines in School-Aged Children.Pediatric allergy, immunology, and pulmonology · 2016Article
- Genetic and epigenetic studies of atopic dermatitis.Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology · 2016Review
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Authors and funding
7 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAsthma is the most common chronic childhood disease. Imbalance of cytokines released from T helper cells and environmental factors, such as exposure to poly-aromatic hydrocarbon (PAH), play pivotal roles in the pathogenesis of asthma. The aim of this study was to compare the mRNA expression patterns of Interleukin (IL)-4, interferon (IFN)-γ and Acyl Co A long chain 3 (ACSL3) in peripheral blood leukocytes of children with and without asthma. To correlate the obtained mRNA data with serum IL-4, IFN-γ and PAH levels. Further, to determine the effect of in vivo exposure to PAH on mRNA expression of IL-4, IFN-γ and ACSL3 genes in a rat model.
methodsA total of 170 children below 16 years (85 pediatric asthma patients and 85 matched healthy controls) were randomly selected from the Riyadh Cohort, Saudi Arabia. Gene expression analysis was performed using qRTPCR. Serum IL-4, IFN-γ and PAH were measured using LINCOplex (human multiplex immunoassay kit) and HPLC respectively.
resultsIL-4 mRNA expression was significantly increased (P < 0.05) in children with asthma compared to healthy control group whereas no differences were observed for either IFN-γ or ACSL3 mRNA. Similarly, serum IL- 4 and PAHs concentration was significantly higher as well in children with asthma in whom IFN-γ was also significantly lower. Results obtained in rats showed that exposure to the benzopyrene prototype PAH resulted in a 2.6 fold (P < 0.001) increased IL-4 mRNA expression in blood.
conclusionPeripheral blood IL-4 mRNA levels, serum concentration of this cytokine are elevated in children with asthma. Also, elevated levels of PAH were observed in children with asthma. Additionally, PAH administration in rodents resulted in an increased IL-4 mRNA which is supposed to partly mediate the inflammatory response noted in asthma.
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