ArticleProceedings of the National Academy of Sciences of the United States of America2014
Digital microfluidic assay for protein detection.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 128 citations in OpenAlex.
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- A portable analog front-end system for label-free sensing of proteins using nanowell array impedance sensors.Scientific reports · 2022Article
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- Thin flexible lab-on-a-film for impedimetric sensing in biomedical applications.Scientific reports · 2022Article
- Label-Free Physical Techniques and Methodologies for Proteins Detection in Microfluidic Biosensor Structures.Biomedicines · 2022Review
- Time-domain signal averaging to improve microparticles detection and enumeration accuracy in a microfluidic impedance cytometer.Biotechnology and bioengineering · 2021Article
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- Biochip with multi-planar electrodes geometry for differentiation of non-spherical bioparticles in a microchannel.Scientific reports · 2021Article
- Multi-frequency impedance sensing for detection and sizing of DNA fragments.Scientific reports · 2021Article
- Development of Gold Nanoparticle Micropatterns for the Electrical Detection of Proteins.Nanomaterials (Basel, Switzerland) · 2021Article
- Potential Microfluidic Devices for COVID-19 Antibody Detection at Point-of-Care (POC): A Review.IEEE sensors journal · 2021Article
- Detection techniques of biological and chemical Hall sensors.RSC advances · 2021Review
- A Low-Cost, Disposable and Portable Inkjet-Printed Biochip for the Developing World.Sensors (Basel, Switzerland) · 2020Article
- Quantitation of Femtomolar-Level Protein Biomarkers Using a Simple Microbubbling Digital Assay and Bright-Field Smartphone Imaging.Angewandte Chemie (International ed. in English) · 2019Review
- Vertically sheathing laminar flow-based immunoassay using simultaneous diffusion-driven immune reactions.RSC advances · 2019Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Global studies of the human proteome have revealed a plethora of putative protein biomarkers. However, their application for early disease detection remains at a standstill without suitable methods to realize their utility in the clinical setting. There thus continues to be tremendous interest in developing new technology for sensitive protein detection that is both low in cost and carries a small footprint to be able to be used at the point of care. The current gold standard method for protein biomarker detection is the ELISA, which measures protein abundance using bulky fluorescent scanners that lack portability. Here, we present a digital microfluidic platform for protein biomarker detection that is low in cost compared with standard optical detection methods, without any compromise in sensitivity. This platform furthermore makes use of simple electronics, enabling its translation into a portable handheld device, and has been developed in a manner that can easily be adapted to assay different types of proteomic biomarkers. We demonstrate its utility in quantifying not only protein abundance, but also activity. Interleukin-6 abundance could be assayed from concentrations as low as 50 pM (an order of magnitude lower than that detectable by a comparable laboratory designed ELISA) using less than 5 μL of sample, and Abelson tyrosine kinase activity was detectable in samples containing 100 pM of kinase.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.