ArticleJournal of lipid research2014
Gangliosides as a potential new class of stem cell markers: the case of GD1a in human bone marrow mesenchymal stem cells.
Article in Journal of lipid research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- FAIMS Shotgun Lipidomics for Enhanced Class- and Charge-State Separation Complemented by Automated Ganglioside Annotation.Analytical chemistry · 2024Article
- Regulation of Glycosylation in Bone Metabolism.International journal of molecular sciences · 2024Review
- Lipid rafts mediate multilineage differentiation of human dental pulp-derived stem cells (DPSCs).Frontiers in cell and developmental biology · 2023Article
- Gangliosides and Their Role in Multilineage Differentiation of Mesenchymal Stem Cells.Biomedicines · 2022Review
- Article
- Ganglioside GD1a enhances osteogenesis by activating ERK1/2 in mesenchymal stem cells ofAging · 2022Article
- Attenuation of Bone Formation through a Decrease in Osteoblasts in Mutant Mice Lacking the GM2/GD2 Synthase Gene.International journal of molecular sciences · 2022Article
- Glycosphingolipids.Advances in experimental medicine and biology · 2021Article
- The Power of LC-MS Based Multiomics: Exploring Adipogenic Differentiation of Human Mesenchymal Stem/Stromal Cells.Molecules (Basel, Switzerland) · 2019Article
- Deletion of Gb3 Synthase in Mice Resulted in the Attenuation of Bone Formation via Decrease in Osteoblasts.International journal of molecular sciences · 2019Article
- Deficiency of GD3 Synthase in Mice Resulting in the Attenuation of Bone Loss with Aging.International journal of molecular sciences · 2019Article
- Fat for fuel: lipid metabolism in haematopoiesis.Clinical & translational immunology · 2019Review
- Particular CSF sphingolipid patterns identify iNPH and AD patients.Scientific reports · 2018Article
- GM1 Ganglioside Promotes Osteogenic Differentiation of Human Tendon Stem Cells.Stem cells international · 2018Article
- GM1 Induced the inflammatory response related to the Raf-1/MEK1/2/ERK1/2 pathway in co-culture of pig mesenchymal stem cells with RAW264.7.Animal cells and systems · 2018Article
- Synthesis and chemical characterization of several perfluorinated sialic acid glycals and evaluation of theirMedChemComm · 2017Article
- Mesenchymal Stem Cells Respond to Hypoxia by Increasing Diacylglycerols.Journal of cellular biochemistry · 2016Article
- Serum Deprivation-Induced Human GM3 Synthase (hST3Gal V) Gene Expression Is Mediated by Runx2 in Human Osteoblastic MG-63 Cells.International journal of molecular sciences · 2015Article
- Phenotypic, Morphological and Adhesive Differences of Human Hematopoietic Progenitor Cells Cultured on Murine versus Human Mesenchymal Stromal Cells.Scientific reports · 2015Article
- Contribution of Glucosylceramide Synthase to the Proliferation of Mouse Osteoblasts.In vivo (Athens, Greece)Article
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Authors and funding
16 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Owing to their exposure on the cell surface and the possibility of being directly recognized with specific antibodies, glycosphingolipids have aroused great interest in the field of stem cell biology. In the search for specific markers of the differentiation of human bone marrow mesenchymal stem cells (hBMSCs) toward osteoblasts, we studied their glycosphingolipid pattern, with particular attention to gangliosides. After lipid extraction and fractionation, gangliosides, metabolically (3)H-labeled in the sphingosine moiety, were separated by high-performance TLC and chemically characterized by MALDI MS. Upon induction of osteogenic differentiation, a 3-fold increase of ganglioside GD1a was observed. Therefore, the hypothesis of GD1a involvement in hBMSCs commitment toward the osteogenic phenotype was tested by comparison of the osteogenic propensity of GD1a-highly expressing versus GD1a-low expressing hBMSCs and direct addition of GD1a in the differentiation medium. It was found that either the high expression of GD1a in hBMSCs or the addition of GD1a in the differentiation medium favored osteogenesis, providing a remarkable increase of alkaline phosphatase. It was also observed that ganglioside GD2, although detectable in hBMSCs by immunohistochemistry with an anti-GD2 antibody, could not be recognized by chemical analysis, likely reflecting a case, not uncommon, of molecular mimicry.
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