ArticleHistochemistry and cell biology2014
Simultaneous detection of multiple targets for ultrastructural immunocytochemistry.
Article in Histochemistry and cell biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed.
- Visualizing suborganellar lipid distribution using correlative light and electron microscopy.Nature cell biology · 2026Article
- An advanced fast method for the evaluation of multiple immunolabelling using gold nanoparticles based on low-energy STEM.Scientific reports · 2024Article
- Joining European Scientific Forces to Face Pandemics.Trends in microbiology · 2021Article
- Imaging VIPER-labeled Cellular Proteins by Correlative Light and Electron Microscopy.Bio-protocol · 2019Article
- Ultrastructural histochemistry in biomedical research: Alive and kicking.European journal of histochemistry : EJH · 2018Article
- ColorEM: analytical electron microscopy for element-guided identification and imaging of the building blocks of life.Histochemistry and cell biology · 2018Review
- Colocalization coefficients evaluating the distribution of molecular targets in microscopy methods based on pointed patterns.Histochemistry and cell biology · 2016Article
- Correlated light and electron microscopy: ultrastructure lights up!Nature methods · 2015Review
- From gross anatomy to the nanomorphome: stereological tools provide a paradigm for advancing research in quantitative morphomics.Journal of anatomy · 2015Article
- The Histochemistry and Cell Biology pandect: the year 2014 in review.Histochemistry and cell biology · 2015Review
- Article
Corrections and comments
- Erratum issued
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Simultaneous detection of biological molecules by means of indirect immunolabeling provides valuable information about their localization in cellular compartments and their possible interactions in macromolecular complexes. While fluorescent microscopy allows for simultaneous detection of multiple antigens, the sensitive electron microscopy immunodetection is limited to only two antigens. In order to overcome this limitation, we prepared a set of novel, shape-coded metal nanoparticles readily discernible in transmission electron microscopy which can be conjugated to antibodies or other bioreactive molecules. With the use of novel nanoparticles, various combinations with commercial gold nanoparticles can be made to obtain a set for simultaneous labeling. For the first time in ultrastructural histochemistry, up to five molecular targets can be identified simultaneously. We demonstrate the usefulness of the method by mapping of the localization of nuclear lipid phosphatidylinositol-4,5-bisphosphate together with four other molecules crucial for genome function, which proves its suitability for a wide range of biomedical applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.