Trial reportLab on a chip2014
An automated integrated platform for rapid and sensitive multiplexed protein profiling using human saliva samples.
Trial report in Lab on a chip, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 61 citations in OpenAlex.
- Role of Salivary Biomarkers in Cystic Fibrosis: A Systematic Review.BioMed research international · 2022Pooled it
- Comparison of classic statistical methods and machine learning approaches to classify readiness.Bioinformatics (Oxford, England) · 2026Article
- Electrochemical monitoring of enzymatic cleavage in nanochannels with nanoparticle-based enhancement: determination of MMP-9 biomarker.Mikrochimica acta · 2023Article
- Advances in Electrochemical Biosensors Based on Nanomaterials for Protein Biomarker Detection in Saliva.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- Microfluidic synthesis of Janus-structured QD-encoded magnetic microbeads for multiplex immunoassay.Mikrochimica acta · 2022Article
- Combined electromechanically driven pulsating flow of nonlinear viscoelastic fluids in narrow confinements.Journal of the Royal Society, Interface · 2022Article
- A narrative review of exploring potential salivary biomarkers in respiratory diseases: still on its way.Journal of thoracic disease · 2021Review
- The predictive power of saliva electrolytes exceeds that of saliva microbiomes in diagnosing early childhood caries.Journal of oral microbiology · 2021Article
- A critical review: Recent advances in "digital" biomolecule detection with single copy sensitivity.Biosensors & bioelectronics · 2021Review
- Ultrasensitive Detection of Attomolar Protein Concentrations by Dropcast Single Molecule Assays.Journal of the American Chemical Society · 2020Article
- Automated Pre-Analytic Processing of Whole Saliva Using Magnet-Beating for Point-of-Care Protein Biomarker Analysis.Micromachines · 2019Article
- Role of Saliva and Salivary Diagnostics in the Advancement of Oral Health.Journal of dental research · 2019Article
- Microfluidic Low-Input Fluidized-Bed Enabled ChIP-seq Device for Automated and Parallel Analysis of Histone Modifications.Analytical chemistry · 2018Article
- Lab-on-chip technology for chronic disease diagnosis.NPJ digital medicine · 2018Review
- Advances in the microbial etiology and pathogenesis of early childhood caries.Molecular oral microbiology · 2017Review
- Point-of-Care Diagnostics: Recent Developments in a Connected Age.Analytical chemistry · 2017Review
- Single-cell profiling of dynamic cytokine secretion and the phenotype of immune cells.PloS one · 2017Article
- Immunoprevalence to Six Waterborne Pathogens in Beachgoers at Boquerón Beach, Puerto Rico: Application of a Microsphere-Based Salivary Antibody Multiplex Immunoassay.Frontiers in public health · 2017Article
- Fluorescence based fiber optic and planar waveguide biosensors. A review.Analytica chimica acta · 2016Review
- Whole-Genome Saliva and Blood DNA Methylation Profiling in Individuals with a Respiratory Allergy.PloS one · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
15 authors at 6 institutions in 2 countries.
Funding
Abstract
During the last decade, saliva has emerged as a potentially ideal diagnostic biofluid for noninvasive testing. In this paper, we present an automated, integrated platform useable by minimally trained personnel in the field for the diagnosis of respiratory diseases using human saliva as a sample specimen. In this platform, a saliva sample is loaded onto a disposable microfluidic chip containing all the necessary reagents and components required for saliva analysis. The chip is then inserted into the automated analyzer, the SDReader, where multiple potential protein biomarkers for respiratory diseases are measured simultaneously using a microsphere-based array via fluorescence sandwich immunoassays. The results are read optically, and the images are analyzed by a custom-designed algorithm. The fully automated assay requires as little as 10 μL of saliva sample, and the results are reported in 70 min. The performance of the platform was characterized by testing protein standard solutions, and the results were comparable to those from the 3.5 h lab bench assay that we have previously reported. The device was also deployed in two clinical environments where 273 human saliva samples collected from different subjects were successfully tested, demonstrating the device's potential to assist clinicians with the diagnosis of respiratory diseases by providing timely protein biomarker profiling information. This platform, which combines noninvasive sample collection and fully automated analysis, can also be utilized in point-of-care diagnostics.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.