Evidence map›Paper›PMID 24448406›Full record

ArticleeLife2014

The splicing regulator PTBP2 controls a program of embryonic splicing required for neuronal maturation.

Qin Li, Sika Zheng, Areum Han, Chia-Ho Lin, Peter Stoilov, Xiang-Dong Fu, Douglas L Black

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in eLife, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 124 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
124citing papers in PubMed, 1 pooled it
7.5field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

124 citing papers in PubMed, 1 synthesis or guideline pooled it, 177 citations in OpenAlex.

  1. Pooled it
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  10. Review
  11. A neuron type-specific microexon inbioRxiv : the preprint server for biology · 2025
    Article
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  17. Base editing ofeLife · 2024
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64 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Qin LiDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, United States.
Sika ZhengDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, United States.
Areum HanDepartment of Bioengineering, Henry Samueli School of Engineering and Applied Science, University of California, Los Angeles, Los Angeles, United States.
Chia-Ho LinDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, United States.
Peter StoilovDepartment of Biochemistry, West Virginia University, School of Medicine, Morgantown, United States.
Xiang-Dong FuDepartment of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, United States.
Douglas L BlackDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, United States.
University of California, Los Angeles · USHoward Hughes Medical Institute · USUniversity of California, San Diego · USWest Virginia University · US

Funding

The Regulation of Neuronal Exon SplicingR01GM049662 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BLACK, DOUGLAS L · 1998 to 2019
$6.5M
Genomic Measurement of Alternative SplicingR01GM084317 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI ARES, MANUEL, YEO, EUGENE WEI-MING · 2009 to 2012
$1.8M
Alternative splicing and nonsense-mediated mRNA decay in neural developmentK99MH096807 · NIMH · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ZHENG, SIKA · 2013 to 2014
$100k
NIGMS NIH HHS R01 GM049662NIGMS NIH HHS R01 GM084317NIGMS NIH HHS R01 GM49662NIMH NIH HHS K99 MH096807
6 · The paper itself

Abstract

We show that the splicing regulator PTBP2 controls a genetic program essential for neuronal maturation. Depletion of PTBP2 in developing mouse cortex leads to degeneration of these tissues over the first three postnatal weeks, a time when the normal cortex expands and develops mature circuits. Cultured Ptbp2(-/-) neurons exhibit the same initial viability as wild type, with proper neurite outgrowth and marker expression. However, these mutant cells subsequently fail to mature and die after a week in culture. Transcriptome-wide analyses identify many exons that share a pattern of mis-regulation in the mutant brains, where isoforms normally found in adults are precociously expressed in the developing embryo. These transcripts encode proteins affecting neurite growth, pre- and post-synaptic assembly, and synaptic transmission. Our results define a new genetic regulatory program, where PTBP2 acts to temporarily repress expression of adult protein isoforms until the final maturation of the neuron. DOI: http://dx.doi.org/10.7554/eLife.01201.001.

Indexed as

Gene Expression RegulationAnimalsCells, CulturedCell SurvivalGene Expression ProfilingGene Knockout TechniquesMiceMolecular Sequence DataNerve Tissue ProteinsNervous SystemNeuronsPolypyrimidine Tract-Binding ProteinSequence Analysis, DNANerve Tissue ProteinsPolypyrimidine Tract-Binding ProteinPtbp2 protein, mousealternative splicinggene regulationneuronal developmentRNA binding protein

Identifiers

PMID24448406
PMCPMC3896118
OpenAlexW2001547716

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.