ArticleScientific reports2014
Micro-a-fluidics ELISA for rapid CD4 cell count at the point-of-care.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
43 citing papers in PubMed, 96 citations in OpenAlex.
- Microfluidic Assays for CD4 T Lymphocyte Counting: A Review.Biosensors · 2025Review
- Development of a C-reactive protein quantification method based on flow rate measurement of an ink solution pushed out by oxygen gas generated by catalase reaction.Mikrochimica acta · 2023Article
- Adaptive time modulation technique for multiplexed on-chip particle detection across scales.Optica · 2023Article
- Review of HIV Self Testing Technologies and Promising Approaches for the Next Generation.Biosensors · 2023Review
- Microfluidic Devices for HIV Diagnosis and Monitoring at Point-of-Care (POC) Settings.Biosensors · 2022Review
- An Electromechanical Lab-on-a-Chip Platform for Colorimetric Detection of Serum Creatinine.ACS omega · 2022Article
- Microparticle-tagged image-based cell counting (ImmunoSpin) for CD4 + T cells.Mikrochimica acta · 2021Article
- A 3D mixing-based portable magnetic device for fully automatic immunofluorescence staining of γ-H2AX in UVC-irradiated CD4RSC advances · 2020Article
- Flow induced particle separation and collection through linear array pillar microfluidics device.Biomicrofluidics · 2020Article
- Development of a Flow-Free Automated Colorimetric Detection Assay Integrated with Smartphone for Zika NS1.Diagnostics (Basel, Switzerland) · 2020Article
- Development of a multiplex fully automated assay for rapid quantification of CD4Biosensors & bioelectronics · 2019Article
- Development of a Flow-Free Magnetic Actuation Platform for an Automated Microfluidic ELISA.RSC advances · 2019Article
- "The Smartphone's Guide to the Galaxy": In Situ Analysis in Space.Biosensors · 2018Review
- Enzyme-catalyzed Ag Growth on Au Nanoparticle-assembled Structure for Highly Sensitive Colorimetric Immunoassay.Scientific reports · 2018Article
- Direct Detection of Unamplified Pathogen RNA in Blood Lysate using an Integrated Lab-in-a-Stick Device and Ultrabright SERS Nanorattles.Scientific reports · 2018Article
- Strategies in Ebola virus disease (EVD) diagnostics at the point of care.Critical reviews in microbiology · 2017Review
- Point-of-care diagnostics to improve maternal and neonatal health in low-resource settings.Lab on a chip · 2017Review
- An integrated double-filtration microfluidic device for isolation, enrichment and quantification of urinary extracellular vesicles for detection of bladder cancer.Scientific reports · 2017Article
- An Assay System for Point-of-Care Diagnosis of Tuberculosis using Commercially Manufactured PCB Technology.Scientific reports · 2017Article
- Paper-based analytical devices for clinical diagnosis: recent advances in the fabrication techniques and sensing mechanisms.Expert review of molecular diagnostics · 2017Review
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 2 countries.
Funding
Abstract
HIV has become one of the most devastating pathogens in human history. Despite fast progress in HIV-related basic research, antiretroviral therapy (ART) remains the most effective method to save AIDS patients' lives. Unfortunately, ART cannot be universally accessed, especially in developing countries, due to the lack of effective treatment monitoring diagnostics. Here, we present an inexpensive, rapid and portable micro-a-fluidic platform, which can streamline the process of an enzyme-linked immunosorbent assay (ELISA) in a fully automated manner for CD4 cell count. The micro-a-fluidic CD4 cell count is achieved by eliminating operational fluid flow via "moving the substrate", as opposed to "flowing liquid" in traditional ELISA or microfluidic methods. This is the first demonstration of capturing and detecting cells from unprocessed whole blood using the enzyme-linked immunosorbent assay (ELISA) in a microfluidic channel. Combined with cell phone imaging, the presented micro-a-fluidic ELISA platform holds great promise for offering rapid CD4 cell count to scale up much needed ART in resource-constrained settings. The developed system can be extended to multiple areas for ELISA-related assays.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.