ArticleNucleic acids research2014
Engineering of a conditional allele reveals multiple roles of XRN2 in Caenorhabditis elegans development and substrate specificity in microRNA turnover.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 35 citations in OpenAlex.
- What goes up must come down: off switches for regulatory RNAs.Genes & development · 2024Review
- Dynamics of miRNA accumulation during C. elegans larval development.Nucleic acids research · 2024Article
- Recent advances in understanding microRNA function and regulation in C. elegans.Seminars in cell & developmental biology · 2024Review
- A germline-targeted genetic screen for xrn-2 suppressors identifies a novel gene C34C12.2 in Caenorhabditis elegans.Genetics and molecular biology · 2023Article
- Observation of conformational changes that underlie the catalytic cycle of Xrn2.Nature chemical biology · 2022Article
- The developmentally timed decay of an essential microRNA family is seed-sequence dependent.Cell reports · 2022Article
- Screening by deep sequencing reveals mediators of microRNA tailing in C. elegans.Nucleic acids research · 2021Article
- Nuclear RNA Regulation by XRN2 and XTBD Family Proteins.Cell structure and function · 2021Review
- microRNA strand selection: Unwinding the rules.Wiley interdisciplinary reviews. RNA · 2021Review
- LSM2-8 and XRN-2 contribute to the silencing of H3K27me3-marked genes through targeted RNA decay.Nature cell biology · 2020Article
- AtXRN4 Affects the Turnover of Chosen miRNA*s inPlants (Basel, Switzerland) · 2020Article
- Terminal uridylyltransferases target RNA viruses as part of the innate immune system.Nature structural & molecular biology · 2018Article
- Recent Molecular Genetic Explorations ofGenetics · 2018Article
- Two distinct transcription termination modes dictated by promoters.Genes & development · 2017Article
- Article
- Removing bias against short sequences enables northern blotting to better complement RNA-seq for the study of small RNAs.Nucleic acids research · 2017Article
- RNA surveillance via nonsense-mediated mRNA decay is crucial for longevity in daf-2/insulin/IGF-1 mutant C. elegans.Nature communications · 2017Article
- MicroRNA Regulation of Oxidative Stress-Induced Cellular Senescence.Oxidative medicine and cellular longevity · 2017Review
- Molting inWorm · 2017Review
- XRN2 Autoregulation and Control of Polycistronic Gene Expresssion in Caenorhabditis elegans.PLoS genetics · 2016Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although XRN2 proteins are highly conserved eukaryotic 5'→3' exonucleases, little is known about their function in animals. Here, we characterize Caenorhabditis elegans XRN2, which we find to be a broadly and constitutively expressed nuclear protein. An xrn-2 null mutation or loss of XRN2 catalytic activity causes a molting defect and early larval arrest. However, by generating a conditionally mutant xrn-2ts strain de novo through an approach that may be also applicable to other genes of interest, we reveal further functions in fertility, during embryogenesis and during additional larval stages. Consistent with the known role of XRN2 in controlling microRNA (miRNA) levels, we can demonstrate that loss of XRN2 activity stabilizes some rapidly decaying miRNAs. Surprisingly, however, other miRNAs continue to decay rapidly in xrn-2ts animals. Thus, XRN2 has unanticipated miRNA specificity in vivo, and its diverse developmental functions may relate to distinct substrates. Finally, our global analysis of miRNA stability during larval stage 1 reveals that miRNA passenger strands (miR*s) are substantially less stable than guide strands (miRs), supporting the notion that the former are mostly byproducts of biogenesis rather than a less abundant functional species.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.