ArticleNucleic acids research2014
Synthetic biology tools for programming gene expression without nutritional perturbations in Saccharomyces cerevisiae.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 107 citations in OpenAlex.
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- Modulating Microbial Materials - Engineering Bacterial Cellulose with Synthetic Biology.ACS synthetic biology · 2024Review
- Designing strong inducible synthetic promoters in yeasts.Nature communications · 2024Article
- Engineered transcription-associated Cas9 targeting in eukaryotic cells.Nature communications · 2024Article
- Engineered transcription-associated Cas9 targeting in eukaryotic cells.bioRxiv : the preprint server for biology · 2024Article
- Improving the Z3EV promoter system to create the strongest yeast promoter.FEMS yeast research · 2024Article
- A Multiplex MoClo Toolkit for Extensive and Flexible Engineering ofACS synthetic biology · 2023Article
- Kar4 is required for the normal pattern of meiotic gene expression.PLoS genetics · 2023Article
- Kar4, the yeast homolog of METTL14, is required for mRNA m6A methylation and meiosis.PLoS genetics · 2023Article
- Multidimensional characterization of inducible promoters and a highly light-sensitive LOV-transcription factor.Nature communications · 2023Article
- Vir1p, the yeast homolog of virilizer, is required for mRNA m6A methylation and meiosis.Genetics · 2023Article
- Chance promoter activities illuminate the origins of eukaryotic intergenic transcriptions.Nature communications · 2023Article
- Titrating Avidity of Yeast-Displayed Proteins Using a Transcriptional Regulator.ACS synthetic biology · 2023Article
- Natural promoters and promoter engineering strategies for metabolic regulation in Saccharomyces cerevisiae.Journal of industrial microbiology & biotechnology · 2023Review
- Vir1p, the Yeast Homolog of Virilizer, is Required for mRNA mbioRxiv : the preprint server for biology · 2023Article
- Kar4, the Yeast Homolog of METTL14, is Required for mRNA mbioRxiv : the preprint server for biology · 2023Article
- Kar4 is Required for the Normal Pattern of Meiotic Gene Expression.bioRxiv : the preprint server for biology · 2023Article
- EVMP: enhancing machine learning models for synthetic promoter strength prediction by Extended Vision Mutant Priority framework.Frontiers in microbiology · 2023Article
- A Toolkit for Precise, Multigene Control inACS synthetic biology · 2022Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
A conditional gene expression system that is fast-acting, is tunable and achieves single-gene specificity was recently developed for yeast. A gene placed directly downstream of a modified GAL1 promoter containing six Zif268 binding sequences (with single nucleotide spacing) was shown to be selectively inducible in the presence of β-estradiol, so long as cells express the artificial transcription factor, Z3EV (a fusion of the Zif268 DNA binding domain, the ligand binding domain of the human estrogen receptor and viral protein 16). We show the strength of Z3EV-responsive promoters can be modified using straightforward design principles. By moving Zif268 binding sites toward the transcription start site, expression output can be nearly doubled. Despite the reported requirement of estrogen receptor dimerization for hormone-dependent activation, a single binding site suffices for target gene activation. Target gene expression levels correlate with promoter binding site copy number and we engineer a set of inducible promoter chassis with different input-output characteristics. Finally, the coupling between inducer identity and gene activation is flexible: the ligand specificity of Z3EV can be re-programmed to respond to a non-hormone small molecule with only five amino acid substitutions in the human estrogen receptor domain, which may prove useful for industrial applications.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.