ArticleJournal of cellular and molecular medicine2014
Serum and supplement optimization for EU GMP-compliance in cardiospheres cell culture.
Article in Journal of cellular and molecular medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 45 citations in OpenAlex.
- The 3D World of Spheroids: Searching for an Optimal Method of Fabricating Pro-Reparative Cardiospheres.International journal of molecular sciences · 2025Article
- Optimizing Wnt activation in fetal calf serum (FCS)-free organoid expansion media.Frontiers in toxicology · 2025Review
- Improved Manufacturing Methods of Extracellular Vesicles Pseudotyped with the Vesicular Stomatitis Virus Glycoprotein.Molecular biotechnology · 2024Article
- Good manufacturing practice production of human corneal limbus-derived stromal stem cells and in vitro quality screening for therapeutic inhibition of corneal scarring.Stem cell research & therapy · 2024Article
- Cytoprotective Effects of Human Platelet Lysate during the Xeno-Free Culture of Human Donor Corneas.International journal of molecular sciences · 2023Article
- The dark side of foetal bovine serum in extracellular vesicle studies.Journal of extracellular vesicles · 2022Review
- Progressive stages of dysmetabolism are associated with impaired biological features of human cardiac stromal cells mediated by the oxidative state and autophagy.The Journal of pathology · 2022Article
- Xeno-free protocol for GMP-compliant manufacturing of human fetal pancreas-derived mesenchymal stem cells.Stem cell research & therapy · 2022Article
- The impact of autophagy modulation on phenotype and survival of cardiac stromal cells under metabolic stress.Cell death discovery · 2022Article
- The dynamic facets of the cardiac stroma: from classical markers to omics and translational perspectives.American journal of translational research · 2022Review
- Lentiviral Vector Production from a Stable Packaging Cell Line Using a Packed Bed Bioreactor.Molecular therapy. Methods & clinical development · 2020Article
- The Microenvironment of Decellularized Extracellular Matrix from Heart Failure Myocardium Alters the Balance between Angiogenic and Fibrotic Signals from Stromal Primitive Cells.International journal of molecular sciences · 2020Article
- Current challenges in three-dimensional bioprinting heart tissues for cardiac surgery.European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery · 2020Review
- Autologous plasma versus fetal calf serum as a supplement for the culture of neutrophils.BMC research notes · 2020Article
- Physiologic expansion of human heart-derived cells enhances therapeutic repair of injured myocardium.Stem cell research & therapy · 2019Article
- Stem Cell Spheroids and Ex Vivo Niche Modeling: Rationalization and Scaling-Up.Journal of cardiovascular translational research · 2017Review
- Endothelial and smooth muscle cells derived from human cardiac explants demonstrate angiogenic potential and suitable for design of cell-containing vascular grafts.Journal of translational medicine · 2017Article
- Sex Differences of Human Cardiac Progenitor Cells in the Biological Response to TNF-Stem cells international · 2017Article
- Normal versus Pathological Cardiac Fibroblast-Derived Extracellular Matrix Differentially Modulates Cardiosphere-Derived Cell Paracrine Properties and Commitment.Stem cells international · 2017Article
- Β-blockers treatment of cardiac surgery patients enhances isolation and improves phenotype of cardiosphere-derived cells.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cardiac progenitor cells (CPCs) isolated as cardiospheres (CSs) and CS-derived cells (CDCs) are a promising tool for cardiac cell therapy in heart failure patients, having CDCs already been used in a phase I/II clinical trial. Culture standardization according to Good Manufacturing Practices (GMPs) is a mandatory step for clinical translation. One of the main issues raised is the use of xenogenic additives (e.g. FBS, foetal bovine serum) in cell culture media, which carries the risk of contamination with infectious viral/prion agents, and the possible induction of immunizing effects in the final recipient. In this study, B27 supplement and sera requirements to comply with European GMPs were investigated in CSs and CDCs cultures, in terms of process yield/efficiency and final cell product gene expression levels, as well as phenotype. B27- free CS cultures produced a significantly reduced yield and a 10-fold drop in c-kit expression levels versus B27+ media. Moreover, autologous human serum (aHS) and two different commercially available GMP AB HSs were compared with standard research-grade FBS. CPCs from all HSs explants had reduced growth rate, assumed a senescent-like morphology with time in culture, and/or displayed a significant shift towards the endothelial phenotype. Among three different GMP gamma-irradiated FBSs (giFBSs) tested, two provided unsatisfactory cell yields, while one performed optimally, in terms of CPCs yield/phenotype. In conclusion, the use of HSs for the isolation and expansion of CSs/CDCs has to be excluded because of altered proliferation and/or commitment, while media supplemented with B27 and the selected giFBS allows successful EU GMP-complying CPCs culture.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.