ArticleMicroscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada2014
A microfluidic-enabled mechanical microcompressor for the immobilization of live single- and multi-cellular specimens.
Article in Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 24 citations in OpenAlex.
- SMORES: a simple microfluidic operating room for the examination and surgery of Stentor coeruleus.Scientific reports · 2024Article
- SMORES: A Simple Microfluidic Operating Room for the Examination and Surgery ofbioRxiv : the preprint server for biology · 2024Article
- A practical reference for studying meiosis in the model ciliateMarine life science & technology · 2022Review
- Cryptic Diversity inMicroorganisms · 2022Article
- The Cilioprotist Cytoskeleton , a Model for Understanding How Cell Architecture and Pattern Are Specified: Recent Discoveries from Ciliates and Comparable Model Systems.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Condensins promote chromosome individualization and segregation during mitosis, meiosis, and amitosis inMolecular biology of the cell · 2018Article
- Toxicity and Applications of Internalised Magnetite Nanoparticles Within LiveBioNanoScience · 2018Article
- Real-time visualization of chromatin modification in isolated nuclei.Journal of cell science · 2017Article
- On chip cryo-anesthesia of Drosophila larvae for high resolution in vivo imaging applications.Lab on a chip · 2017Article
- Refinements of and commentary on the silver staining techniques of Fernández-Galiano.Biotechnic & histochemistry : official publication of the Biological Stain Commission · 2016Article
- A mechanical microcompressor for high resolution imaging of motile specimens.Experimental cell research · 2015Article
- Use of a novel cell adhesion method and digital measurement to show stimulus-dependent variation in somatic and oral ciliary beat frequency in Paramecium.The Journal of eukaryotic microbiologyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
17 authors at 3 institutions in 1 country.
Funding
Abstract
A microcompressor is a precision mechanical device that flattens and immobilizes living cells and small organisms for optical microscopy, allowing enhanced visualization of sub-cellular structures and organelles. We have developed an easily fabricated device, which can be equipped with microfluidics, permitting the addition of media or chemicals during observation. This device can be used on both upright and inverted microscopes. The apparatus permits micrometer precision flattening for nondestructive immobilization of specimens as small as a bacterium, while also accommodating larger specimens, such as Caenorhabditis elegans, for long-term observations. The compressor mount is removable and allows easy specimen addition and recovery for later observation. Several customized specimen beds can be incorporated into the base. To demonstrate the capabilities of the device, we have imaged numerous cellular events in several protozoan species, in yeast cells, and in Drosophila melanogaster embryos. We have been able to document previously unreported events, and also perform photobleaching experiments, in conjugating Tetrahymena thermophila.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.