ArticleThe New phytologist2014
Simultaneous profiling of seed-associated bacteria and fungi reveals antagonistic interactions between microorganisms within a shared epiphytic microbiome on Triticum and Brassica seeds.
Article in The New phytologist, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
80 citing papers in PubMed, 179 citations in OpenAlex.
- Review
- Study on the diversity, structure, and function of endophytic bacteria in seeds of genuine medicinal plants in gansu province.BMC plant biology · 2026Article
- What do we know about the seed microbiome?Microbiome · 2026Review
- The bark latent fungus Botryosphaeria dothidea exacerbates branch dieback following the infection with Verticillium dahliae.Stress biology · 2026Article
- Elevation shapes the seed endophytic bacteria richness and composition ofAIMS microbiology · 2026Article
- Exploring the Bacterial Microbiota of Seeds.Microbial biotechnology · 2025Review
- Harnessing Seed Endophytic Microbiomes: A Hidden Treasure for Enhancing Sustainable Agriculture.Plants (Basel, Switzerland) · 2025Review
- Defining the cultured and uncultured bacterial fractions in Cannabis seeds.Environmental microbiome · 2025Article
- The seed microbiota from an application perspective: an underexplored frontier in plant-microbe interactions.Crop health · 2025Review
- Seed banking impacts native Acacia ulicifolia seed microbiome composition and function.Environmental microbiome · 2025Article
- Seed-mediated vertical transmission of Pantoea core endophytes.The ISME journal · 2025Article
- Host genotype-driven shifts in theFrontiers in microbiology · 2025Article
- Dynamics of endophytic fungi composition inFrontiers in microbiology · 2025Article
- Article
- Wild again: recovery of a beneficial Cannabis seed endophyte from low domestication genotypes.Microbiome · 2024Article
- Impact of thermal seed treatment on spermosphere microbiome, metabolome and viability of winter wheat.Scientific reports · 2024Article
- Characterization of differences in seed endophytic microbiome in conventional and organic rice by amplicon-based sequencing and culturing methods.Microbiology spectrum · 2024Article
- Endosphere mycobiome in mature rice roots originate from both seedlings and soils.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Heterosis of endophytic microbiomes in hybrid rice varieties improves seed germination.mSystems · 2024Article
- Exploring Cereal Metagenomics: Unravelling Microbial Communities for Improved Food Security.Microorganisms · 2024Review
20 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In order to address the hypothesis that seeds from ecologically and geographically diverse plants harbor characteristic epiphytic microbiota, we characterized the bacterial and fungal microbiota associated with Triticum and Brassica seed surfaces. The total microbial complement was determined by amplification and sequencing of a fragment of chaperonin 60 (cpn60). Specific microorganisms were quantified by qPCR. Bacteria and fungi corresponding to operational taxonomic units (OTU) that were identified in the sequencing study were isolated and their interactions examined. A total of 5477 OTU were observed from seed washes. Neither total epiphytic bacterial load nor community richness/evenness was significantly different between the seed types; 578 OTU were shared among all samples at a variety of abundances. Hierarchical clustering revealed that 203 were significantly different in abundance on Triticum seeds compared with Brassica. Microorganisms isolated from seeds showed 99-100% identity between the cpn60 sequences of the isolates and the OTU sequences from this shared microbiome. Bacterial strains identified as Pantoea agglomerans had antagonistic properties toward one of the fungal isolates (Alternaria sp.), providing a possible explanation for their reciprocal abundances on both Triticum and Brassica seeds. cpn60 enabled the simultaneous profiling of bacterial and fungal microbiota and revealed a core seed-associated microbiota shared between diverse plant genera.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.