ArticleMolecular microbiology2014
Building the bacterial orisome: high-affinity DnaA recognition plays a role in setting the conformation of oriC DNA.
Article in Molecular microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 47 citations in OpenAlex.
- Robust helicase loading mechanism underlies diverse architectures of bacterial replication origins.Nucleic acids research · 2026Article
- Review
- DnaA modulates the gene expression and morphology of the Lyme disease spirochete.bioRxiv : the preprint server for biology · 2024Article
- Single-stranded DNA recruitment mechanism in replication origin unwinding by DnaA initiator protein and HU, an evolutionary ubiquitous nucleoid protein.Nucleic acids research · 2023Article
- Borrelia burgdorferi DnaA and the Nucleoid-Associated Protein EbfC Coordinate Expression of theJournal of bacteriology · 2023Article
- Concerted actions of DnaA complexes with DNA-unwinding sequences within and flanking replication origin oriC promote DnaB helicase loading.The Journal of biological chemistry · 2022Article
- Bacterial cell proliferation: from molecules to cells.FEMS microbiology reviews · 2021Review
- Blocking, Bending, and Binding: Regulation of Initiation of Chromosome Replication During theFrontiers in microbiology · 2021Review
- Mechanisms of Theta Plasmid Replication in Enterobacteria and Implications for Adaptation to Its Host.EcoSal Plus · 2020Review
- Blocking the Trigger: Inhibition of the Initiation of Bacterial Chromosome Replication as an Antimicrobial Strategy.Antibiotics (Basel, Switzerland) · 2019Review
- The bacterial cell cycle, chromosome inheritance and cell growth.Nature reviews. Microbiology · 2019Review
- Identification of a basal system for unwinding a bacterial chromosome origin.The EMBO journal · 2019Article
- Crosstalk Regulation Between Bacterial Chromosome Replication and Chromosome Partitioning.Frontiers in microbiology · 2019Review
- Changing Perspectives on the Role of DnaA-ATP in Orisome Function and Timing Regulation.Frontiers in microbiology · 2019Review
- DNA gyrase activity regulates DnaA-dependent replication initiation in Bacillus subtilis.Molecular microbiology · 2018Article
- Low Affinity DnaA-ATP Recognition Sites inFrontiers in microbiology · 2018Article
- Regulatory dynamics in the ternary DnaA complex for initiation of chromosomal replication in Escherichia coli.Nucleic acids research · 2017Article
- Review
- Review
- The DnaA Cycle inFrontiers in microbiology · 2017Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
During assembly of the E. coli pre-replicative complex (pre-RC), initiator DnaA oligomers are nucleated from three widely separated high-affinity DnaA recognition sites in oriC. Oligomer assembly is then guided by low-affinity DnaA recognition sites, but is also regulated by a switch-like conformational change in oriC mediated by sequential binding of two DNA bending proteins, Fis and IHF, serving as inhibitor and activator respectively. Although their recognition sites are separated by up to 90 bp, Fis represses IHF binding and weak DnaA interactions until accumulating DnaA displaces Fis from oriC. It remains unclear whether high-affinity DnaA binding plays any role in Fis repression at a distance and it is also not known whether all high-affinity DnaA recognition sites play an equivalent role in oligomer formation. To examine these issues, we developed origin-selective recombineering methods to mutate E. coli chromosomal oriC. We found that, although oligomers were assembled in the absence of any individual high-affinity DnaA binding site, loss of DnaA binding at peripheral sites eliminated Fis repression, and made binding of both Fis and IHF essential. We propose a model in which interaction of DnaA molecules at high-affinity sites regulates oriC DNA conformation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.