Evidence map›Paper›PMID 24443677›Full record

ArticleProceedings. IEEE International Symposium on Biomedical Imaging2012

3D single molecule tracking and superresolution microscopy using multifocal plane microscopy.

Sripad Ram, E Sally Ward, Raimund J Ober

Abstract read
In one paragraph

Article in Proceedings. IEEE International Symposium on Biomedical Imaging, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Sripad RamDepartment of Immunology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
E Sally WardDepartment of Immunology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
Raimund J OberDepartment of Immunology, University of Texas Southwestern Medical Center, Dallas, TX, USA; Department of Electrical Engineering, University of Texas at Dallas, Richardson, TX, USA.

Funding

High accuracy quantum dot tracking in live cellsR01GM085575 · NIGMS · UNIVERSITY OF TEXAS DALLAS · PI OBER, RAIMUND J · 2008 to 2016
$2.5M
NIGMS NIH HHS R01 GM085575
6 · The paper itself

Abstract

The study of cellular processes in three-dimensions is severely limited by the lack of imaging methodologies that allow for fast 3D tracking of cellular events and 3D superresolution imaging of sub-cellular structures. We have developed a 3D imaging modality, multifocal plane microscopy (MUM), that provides a powerful approach for 3D single molecule tracking and 3D superresolution microscopy. Here we review the technical challenges associated with 3D single molecule localization that is fundamental to both 3D tracking and 3D superresolution, and discuss how MUM overcomes these problems.

Indexed as

2D/3D localization accuracyCramer-Rao lower boundFisher information matrixmaximum likelihood estimatorresolution limits

Identifiers

PMID24443677
PMCPMC3892903

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.