ArticleThe Journal of biological chemistry2014
Structure and biochemical properties of the alkene producing cytochrome P450 OleTJE (CYP152L1) from the Jeotgalicoccus sp. 8456 bacterium.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 192 citations in OpenAlex.
- Engineering peroxygenases for pharmaceutical applications: recent advances and future perspectives.Chemical science · 2026Review
- Article
- Anchoring mechanism-inspired discovery of a bacterial P450 gene conferring resistance to auxin herbicides.Nature communications · 2026Article
- Regulation of ferryl reactivity by the cytochrome P450 decarboxylase OleT.Journal of inorganic biochemistry · 2025Article
- Structural insights into S-lignin O-demethylation via a rare class of heme peroxygenase enzymes.Nature communications · 2025Article
- Coordinated conformational changes in P450 decarboxylases enable hydrocarbons production from renewable feedstocks.Nature communications · 2025Article
- Exploring 1-alkene biosynthesis in bacterial antagonists and Jeotgalicoccus sp. ATCC 8456.FEMS microbiology letters · 2025Article
- In Vitro Characterization of Kitasetaline Biosynthesis Reveals a Bifunctional P450 Decarboxylase and a Vinyl β-Carboline Intermediate Susceptible to Nonenzymatic Thiol Addition.Journal of the American Chemical Society · 2024Article
- On the engineering of reductase-based-monooxygenase activity in CYP450 peroxygenases.Chemical science · 2024Article
- Functional Model of Compound II of Cytochrome P450: Spectroscopic Characterization and Reactivity Studies of a FeJACS Au · 2024Article
- Biosynthesis pathways of expanding carbon chains for producing advanced biofuels.Biotechnology for biofuels and bioproducts · 2023Review
- Dimer-assisted mechanism of (un)saturated fatty acid decarboxylation for alkene production.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Design of a HProtein science : a publication of the Protein Society · 2022Article
- Light-driven biocatalytic oxidation.Chemical science · 2022Review
- Current state and future perspectives of cytochrome P450 enzymes for C-H and C=C oxygenation.Synthetic and systems biotechnology · 2022Article
- Microbial pathways for advanced biofuel production.Biochemical Society transactions · 2022Review
- Hemoprotein Catalyzed Oxygenations: P450s, UPOs, and Progress toward Scalable Reactions.JACS Au · 2021Review
- Quantum Mechanical Investigation of the Oxidative Cleavage of the C-C Backbone Bonds in Polyethylene Model Molecules.Polymers · 2021Article
- Product Distributions of Cytochrome P450 OleTInternational journal of molecular sciences · 2021Article
- A Photoclick-Based High-Throughput Screening for the Directed Evolution of Decarboxylase OleT.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Article
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Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
Abstract
The production of hydrocarbons in nature has been documented for only a limited set of organisms, with many of the molecular components underpinning these processes only recently identified. There is an obvious scope for application of these catalysts and engineered variants thereof in the future production of biofuels. Here we present biochemical characterization and crystal structures of a cytochrome P450 fatty acid peroxygenase: the terminal alkene forming OleTJE (CYP152L1) from Jeotgalicoccus sp. 8456. OleTJE is stabilized at high ionic strength, but aggregation and precipitation of OleTJE in low salt buffer can be turned to advantage for purification, because resolubilized OleTJE is fully active and extensively dissociated from lipids. OleTJE binds avidly to a range of long chain fatty acids, and structures of both ligand-free and arachidic acid-bound OleTJE reveal that the P450 active site is preformed for fatty acid binding. OleTJE heme iron has an unusually positive redox potential (-103 mV versus normal hydrogen electrode), which is not significantly affected by substrate binding, despite extensive conversion of the heme iron to a high spin ferric state. Terminal alkenes are produced from a range of saturated fatty acids (C12-C20), and stopped-flow spectroscopy indicates a rapid reaction between peroxide and fatty acid-bound OleTJE (167 s(-1) at 200 μm H2O2). Surprisingly, the active site is highly similar in structure to the related P450BSβ, which catalyzes hydroxylation of fatty acids as opposed to decarboxylation. Our data provide new insights into structural and mechanistic properties of a robust P450 with potential industrial applications.
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