ArticleJournal of bacteriology2014
Salinity-dependent impacts of ProQ, Prc, and Spr deficiencies on Escherichia coli cell structure.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
22 citing papers in PubMed, 29 citations in OpenAlex.
- Peptidoglycan remodeling improves salt resilience ofApplied and environmental microbiology · 2026Article
- Structural dependence of ProQ-mRNA interactions in live bacterial cells.Communications biology · 2025Article
- Different RNA recognition by ProQ and FinO depends on the sequence surrounding intrinsic terminator hairpins.RNA (New York, N.Y.) · 2025Article
- The RNA-binding protein ProQ directly binds and regulates virulence genes in enterohemorrhagicFrontiers in cellular and infection microbiology · 2025Article
- Improved RNA stability estimation through Bayesian modeling reveals mostProceedings of the National Academy of Sciences of the United States of America · 2024Article
- The FinO/ProQ-like protein PA2582 impacts antimicrobial resistance inFrontiers in microbiology · 2024Article
- Biochemical and genetic dissection of the RNA-binding surface of the FinO domain ofRNA (New York, N.Y.) · 2023Article
- Biochemical and genetic dissection of the RNA-binding surface of the FinO domain ofbioRxiv : the preprint server for biology · 2023Article
- FinO/ProQ-family proteins: an evolutionary perspective.Bioscience reports · 2023Review
- The RNA-Binding Protein ProQ Impacts Exopolysaccharide Biosynthesis and Second Messenger Cyclic di-GMP Signaling in the Fire Blight Pathogen Erwinia amylovora.Applied and environmental microbiology · 2022Article
- The Role and Targets of the RNA-Binding Protein ProQ in the Gram-Negative Bacterial Pathogen Pasteurella multocida.Journal of bacteriology · 2022Article
- Article
- New sequencing methodologies reveal interplay between multiple RNA-binding proteins and their RNAs.Current genetics · 2020Review
- RNA-RNA Interactomes of ProQ and Hfq Reveal Overlapping and Competing Roles.Molecular cell · 2020Article
- Transposon-insertion sequencing screens unveil requirements for EHEC growth and intestinal colonization.PLoS pathogens · 2019Article
- Imaging Bacterial Cell Wall Biosynthesis.Annual review of biochemistry · 2018Review
- ProQ/FinO-domain proteins: another ubiquitous family of RNA matchmakers?Molecular microbiology · 2017Review
- Structure of theRNA (New York, N.Y.) · 2017Article
- Silencing of natural transformation by an RNA chaperone and a multitarget small RNA.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Regulated proteolysis of a cross-link-specific peptidoglycan hydrolase contributes to bacterial morphogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
ProQ is a cytoplasmic protein with RNA chaperone activities that reside in FinO- and Hfq-like domains. Lesions at proQ decrease the level of the osmoregulatory glycine betaine transporter ProP. Lesions at proQ eliminated ProQ and Prc, the periplasmic protease encoded by the downstream gene prc. They dramatically slowed the growth of Escherichia coli populations and altered the morphologies of E. coli cells in high-salinity medium. ProQ and Prc deficiencies were associated with different phenotypes. ProQ-deficient bacteria were elongated unless glycine betaine was provided. High-salinity cultures of Prc-deficient bacteria included spherical cells with an enlarged periplasm and an eccentric nucleoid. The nucleoid-containing compartment was bounded by the cytoplasmic membrane and peptidoglycan. This phenotype was not evident in bacteria cultivated at low or moderate salinity, nor was it associated with murein lipoprotein (Lpp) deficiency, and it differed from those elicited by the MreB inhibitor A-22 or the FtsI inhibitor aztreonam at low or high salinity. It was suppressed by deletion of spr, which encodes one of three murein hydrolases that are redundantly essential for enlargement of the murein sacculus. Prc deficiency may alter bacterial morphology by impairing control of Spr activity at high salinity. ProQ and Prc deficiencies lowered the ProP activity of bacteria cultivated at moderate salinity by approximately 70% and 30%, respectively, but did not affect other osmoregulatory functions. The effects of ProQ and Prc deficiencies on ProP activity are indirect, reflecting their roles in the maintenance of cell structure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.