ArticleMolecular human reproduction2014
Patch clamp studies of human sperm under physiological ionic conditions reveal three functionally and pharmacologically distinct cation channels.
Article in Molecular human reproduction, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
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Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it, 60 citations in OpenAlex.
- The Catsper channel and its roles in male fertility: a systematic review.Reproductive biology and endocrinology : RB&E · 2017Pooled it
- Off-Target Activation of CatSper Limits the Utility of a Novel Small Molecule Slo3 Inhibitor and its Derivatives in Human Sperm.ACS pharmacology & translational science · 2026Article
- Membrane potential and feedback dynamics regulate CatSper-mediated progesterone signaling in human sperm.bioRxiv : the preprint server for biology · 2025Article
- Comparison between Inhibition of CatSper and KSper Channels with NNC 55-0396 and Quinidine on Human Sperm Function.Iranian journal of medical sciences · 2025Article
- Review
- Calcium Channels and Modulators as Potential Therapeutic Targets for Contraceptives and Male Fertility: A Scoping Review.Open access journal of contraception · 2025Review
- The sperm-specific KFrontiers in cell and developmental biology · 2024Article
- Effects of in vitro repaglinide supplementation on improving sperm motility parameters, viability, and DNA integrity in normozoospermic and asthenozoospermic men.JBRA assisted reproduction · 2023Article
- SLO3: A Conserved Regulator of Sperm Membrane Potential.International journal of molecular sciences · 2023Review
- The Role of Sperm Membrane Potential and Ion Channels in Regulating Sperm Function.International journal of molecular sciences · 2023Review
- A selective inhibitor of the sperm-specific potassium channel SLO3 impairs human sperm function.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Electrophysiology and Fluorescence Spectroscopy Approaches for Evaluating Gamete and Embryo Functionality in Animals and Humans.Biomolecules · 2022Review
- The antidepressant Sertraline inhibits CatSper Ca2+ channels in human sperm.Human reproduction (Oxford, England) · 2021Article
- Recording Electrical Currents across the Plasma Membrane of Mammalian Sperm Cells.Journal of visualized experiments : JoVE · 2021Article
- HVCN1 but Not Potassium Channels Are Related to Mammalian Sperm Cryotolerance.International journal of molecular sciences · 2021Article
- KCNQ1 Potassium Channel Expressed in Human Sperm Is Involved in Sperm Motility, Acrosome Reaction, Protein Tyrosine Phosphorylation, and Ion Homeostasis During Capacitation.Frontiers in physiology · 2021Article
- Sperm ion channels and transporters in male fertility and infertility.Nature reviews. Urology · 2021Review
- Elucidating the Role of KInternational journal of molecular sciences · 2019Article
- Human sperm ion channel (dys)function: implications for fertilization.Human reproduction update · 2019Article
- Continuous behavioural 'switching' in human spermatozoa and its regulation by Ca2+-mobilising stimuli.Molecular human reproduction · 2019Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Whilst fertilizing capacity depends upon a K(+) conductance (GK) that allows the spermatozoon membrane potential (Vm) to be held at a negative value, the characteristics of this conductance in human sperm are virtually unknown. We therefore studied the biophysical/pharmacological properties of the K(+) conductance in spermatozoa from normal donors held under voltage/current clamp in the whole cell recording configuration. Our standard recording conditions were designed to maintain quasi-physiological, Na(+), K(+) and Cl(-) gradients. Experiments that explored the effects of ionic substitution/ion channel blockers upon membrane current/potential showed that resting Vm was dependent upon a hyperpolarizing K(+) current that flowed via channels that displayed only weak voltage dependence and limited (∼7-fold) K(+) versus Na(+) selectivity. This conductance was blocked by quinidine (0.3 mM), bupivacaine (3 mM) and clofilium (50 µM), NNC55-0396 (2 µM) and mibefradil (30 µM), but not by 4-aminopyridine (2 mM, 4-AP). Progesterone had no effect upon the hyperpolarizing K(+) current. Repolarization after a test depolarization consistently evoked a transient inward 'tail current' (ITail) that flowed via a second population of ion channels with poor (∼3-fold) K(+) versus Na(+) selectivity. The activity of these channels was increased by quinidine, 4-AP and progesterone. Vm in human sperm is therefore dependent upon a hyperpolarizing K(+) current that flows via channels that most closely resemble those encoded by Slo3. Although 0.5 µM progesterone had no effect upon these channels, this hormone did activate the pharmacologically distinct channels that mediate ITail. In conclusion, this study reveals three functionally and pharmacologically distinct cation channels: Ik, ITail, ICatSper.
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